辐照氟银脐动脉的研制及抑菌效果观察

The Mechanical Behavior and Inmuological Level and Bacteriostatic Effect of Lrradiated Human Umbilical Atery Incorporated with Nofloxacin and Silver

目的:观察辐照氟银处理脐动脉的抑菌效果。方法:在无菌条件下剥离健康新生儿脐动脉并将其随机分为4组,即新鲜脐动脉组(Ⅰ组),冷冻组(Ⅱ组)、辐照组(Ⅲ组)、辐照氟银组(Ⅳ组)。对辐照氟银组脐动脉进行含银量测量、细菌培养及抑菌实验并与其他3组进行比较。结果:辐照氟银脐动脉组含银(249.7±16.8)μg/g,细菌培养无细菌生长,抑菌实验证实辐照氟银脐动脉具有抑菌作用,与其他各组相比差别显著(P<0.01)。结论:辐照氟银脐动脉富含抗菌物质(Ag+),体外抑菌实验证实具有明显抗菌作用,可用来预防移植后感染导致的手术失败。

Objective. To test the mechanical behavior and inmunological level and the bacteriostatic effect of Irradiated Human Umbilical Atery Incorporated withNofloxacin and Silver（IHUVINS）. Methods： Human Umbilical Atery was randomly divided into four groups： fresh Human UmbilicalAtery（groupⅠ）, deepen-frozen Human Umbilical Atery （group Ⅱ）, Lyophilize-irradiated Human Umbilical Atery （group Ⅲ） ,Irradiated Human Umbilical Atery Incorporated withNofloxacin and Silver （group IV）; Ag^＋ Level was detected in the IHUVINS group and all groups were assayde by bacterium culture and inhibiting bacteria in vitro. Results：Ag^＋ was detected in the IHUVINS and no bacterial growth was found in group I and IV. By contrast,the IHUVINS effectively inhibited growth of bacteria（P〈0.01）. Conclusion： Irradiated Human Umbilical Venous Incorporated with Nofloxacin has good bacteriostatic effect and can be used to avoid the failure because of ifection and cross-infection after transplantation.