2004-2005年南昌地区淋病奈瑟菌质粒谱型研究

Study on the Plasmid Profiles of Neisseria Gonorrhoeae Isolated in Nanchang During 2004-2005

目的:研究南昌地区淋病奈瑟菌质粒谱型的分布情况。方法:(1)采用碱裂解法提取淋病奈瑟菌质粒;(2)采用琼脂糖电泳法测定质粒分子量大小,并对菌株的青霉素耐药现象与4.5 Md质粒的关系进行分析。结果:218株淋病奈瑟菌中有206株菌携带质粒,质粒总检出率为94.50%;质粒谱共分7型,以4.5 Md+2.6 Md和24.5 Md+4.5 Md+2.6 Md为主,分别占30.73%及24.77%,未检出3.2 Md的R质粒。结论:南昌地区淋病奈瑟菌质粒谱型的分析对该地区淋病的防治有重要意义;4.5 Md质粒与青霉素耐药密切相关。

Objective： To survey plasmid profiles （PP）of Neisseria gonorrhoeae in Nanchang. Methods：（1） The plasmids of N. gonorrhoeae were extracted by alkaline lysis technique, and then analyzed for the distribution of PP. （2）The relationship between penicillin-resistance and resistant plasmid were discussed. Results： Two hundred and six strains of N. gonorrhoeae harboured plasmids and the detected percentage accounted for 94. 50%. There were 7 types of PP. The PP types of 4.5 Md＋2.6 Md（30. 73%） and 24.5 Md＋4.5 Md＋2.6 Md（24.77%） were prominent, 3.2 Md resistant plasmid was not detected. Conclusion： The study of plasmid profiles of Neisseria gonorrhoeae in Nanchang would be helpful in treatment and control of Gonorrhea. The resistant plasmid is 4.5 Md ＂Asia type＂ plasmid.