致病性镰刀菌的体外药敏实验研究

Antifungal susceptibility test for pathogenic Fusarium spp in vitro

目的：测定几种抗真菌药物对致病性镰刀菌的最小抑菌浓度，了解镰刀菌对这几种药物的敏感性，为临床治疗提供依据。方法：实验菌株为北京大学真菌和真菌病研究中心保存的23株镰刀菌和同仁医院眼科研究所提供的临床分离的68株镰刀菌。将受试镰刀菌株在PDA上25℃培养7-10d后，参照NCCLS M38-P方案，用两性霉素B、伊曲康唑、那他霉素、特比萘芬等4种抗真菌药物对91株临床分离的致病性镰刀菌进行MIC测定。结果：两性霉素B和那他霉索对茄病镰刀菌和串珠镰刀菌的MIC相对较低，伊曲康唑对茄病镰刀菌和串珠镰刀菌的MIC均较高，而特比萘芬对茄病镰刀菌的MIC值高于串珠镰刀菌。结论：两性霉素B与那他霉素对茄病镰刀菌和串珠镰刀菌体外抑菌作用相对较强。而伊曲康唑和特比萘芬不够敏感。

Objective： To analyze MICs of four kinds of antifungal drugs against the common pathogenic Fusarium spp. in order to provide reference for clinical therapy. Methods： The strains tested were cultured on the PDA at 25℃ for 7- 10 days. Results： The MICs of amphotericin B and natamycin against F. solani and F. moniliforme were relatively lower, the MICs of itraconazole agaist F. solani and F. moniliforme were higher, and the MICs of terhinsfine agninst F. solani were higher than those against F. moniliforme. According to the NCCLS M38 - P, the MICs of four antifungal drugs including amphotericin B, itraconazole, natamycin and terhinafine were tested against the 91 strains of Fusarium spp. Conclusion： Amphotericin B and natamycin have better inhibitory effects against F. solani and F. moniliforme in vitro, while itraconazole and terhinafine have lower inhibition.