芦荟凝胶抑制大鼠Ⅱ度放射性皮炎发生的机理研究

Aloe gel depresses the occurrence of dermatitis vesicular reaction by radiation in rats

目的:在分子水平探讨芦荟凝胶抑制大鼠Ⅱ度放射性皮炎发生的机理。方法:首先建立SD大鼠裸露背部10%Ⅱ度放射性皮炎模型,随机分成两组,对照组为自然愈合组,实验组为芦荟外涂组,实验组每日进行鲜芦荟凝胶对辐射伤口区的外涂,每日两次。辐射伤后2、6、10、18、24、34d各活杀大鼠10只,每组各5只。采用免疫组织化学方法并利用图像分析技术,分别记录伤口局部ICAM-1、EGF和bFGF的表达量。结果:实验组ICAM-1的表达总体均数低于对照组的表达P<0.01,差异有显著意义。实验组EGF和bFGF的表达总体均数高于对照组的表达,P<0.05,差异有显著意义。结论:芦荟凝胶对大鼠Ⅱ度放射性皮炎的发生有抑制作用,机理与ICAM-1分泌的抑制及EGF,bFGF分泌的促进相关。

Objective ：To study the mechanism of aloe gel on depression of radioactive dermatitis vesicular reaction in rats at molecular level. Methods. The rats models of 10% H radioactive dermatitis vesicular reaction on nude back were established. The rats were divided into 2 groups randomly： the normal healing group was the control group, and experimental group treated with aloe gel twice a day. At the 2th, 6th, 10th, 18th, 24th and 34th day after radiation, 5 rats in each group were killed, the protein of ICM- 1, EGF and bFGF were stained by immunohistochemistry and image analysis. Results： The total expressions of ICM-1 in experimental groups was significantly lower than that in control groups（P〈0. O1 ）. The total expression of EGF and bFGF in experimental group were significantly higher than that of control group（P〈O. 05 ）. Conclusion： The aloe gel could depress radioactive dermatitis vesicular reaction by increasing the EGF, bFGF release and decrease ICAM-1 secretion.