大肠癌患者手术前后外周血淋巴细胞CD44的表达

Expression of peripheral blood CD44 in patients with colon cancer

目的：研究大肠癌患者手术前后细胞表面黏附分子CD44在外周血淋巴细胞中的表达及临床意义。方法：根治术的大肠癌患者39例，男24例，女15例，年龄30岁-57岁，平均39岁。应用流式细胞免疫学方法检测外周血淋巴细胞中CD44的表达，并与正常对照（n=25）进行比较。结果：大肠癌患者外周血淋巴细胞中CD44的表达均明显高于正常对照（407．1±12．3VS321.7±15．1，P〈0.01），手术后2周（351.2±15．4）和12个月（345.1±18．2）表达降低，但仍高于对照（P〈0.05）。大肠癌中晚期CD44的表达明显高于早期（439.7±21．6 vs 393.2±17．5，P〈0.05），大肠癌有转移患者CD44表达均高于无转移（432.1±16．2vs 398.1±16．6，P〈0.01），但不同组织类型的大肠癌CD44表达无显著性差异（P〉0.05）。结论：大肠癌患者外周血淋巴细胞中CD44的表达水平明显高于正常对照，且手术后可以明显下降，其表达水平与大肠癌的进展情况及有无转移、预后有一定关系。

Objective：To investigate the expression of peripheral blood CD44 in colon cancer. Methods： Data of forty-nine patients （ male 24, female 15, age ranging from 30 to 57 ） with colon cancer undergoing gastrectomy were reviewed, and 25 normal donors served as controls. The expression of peripheral blood CIM4 detected by flow-cytometry. Results： The peripheral blood lymphocyte CIM4 contents in patients with colon cancer were significantly higher than those in normal controls （407.1±12.3 vs 321.7±15.1, P〈0.01 ）, and CIM4 lower （P〈0.05 ） after operation in 2 weeks （351.2± 15.4） and 12 months （345.1±18.2）. Distinctly statistic difference was showed between CIM4 in advanced stage and early stages （439.7 ±21.6 vs 393.2±17.5, P〈0.05） ; colon cancer with metastasis or distance metastasis showed higher expression of CD4 than that without metastasis or distance metastasis （432.1±16.2 vs 398.1±16.6, P〈0.01 ）. No difference in CD4 contents was observed in different histopathological types in colon cancer. （ P〉0.05 ）. Conclusion： The peripheral blood lymphocyte CD44 contents in colon cancer are significantly higher, and CD4 lower after operation. The CD4 expression in colon cancer might be an important means to differentiate benign from malignant renal tumor, and to detect invasive or metastasis and estimate prognosis of colon cancer.