姜黄素对子宫颈癌HeLa细胞增殖抑制作用及其机制的研究

Effect of anti-proliferation of curcumin on cervical carcinoma HeLa cells and its mechanism

目的:探讨姜黄素(Curcum in)对体外培养人子宫颈癌HeLa细胞抗癌作用及分子机制。方法:MTT法检测细胞增殖,流式细胞仪检测凋亡和细胞周期,PI/Hoechst33258荧光双染法检测细胞凋亡,W estern b lot法检测细胞凋亡相关蛋白Bc l-2和Bax蛋白的表达。结果:姜黄素对HeLa细胞生长有抑制作用,并呈剂量依赖性;流式细胞仪分析证实姜黄素能使HeLa细胞阻滞在S期,并出现亚二倍体凋亡峰;荧光双染法可见凋亡细胞;W estern b lot结果显示Bax蛋白表达均上调,而Bc l-2的表达无明显影响。结论:姜黄素对人子宫颈癌HeLa细胞的增殖具有显著的抑制作用并可诱导细胞凋亡;Bax蛋白的表达上调可能参与了诱导细胞凋亡。

Objective. To investigate the mechanism of anti-tumor effects of cureumin on cervical carcinoma HeLa cells. Methods： Growth inhibition rates of HeLa cells were measured with MTF method. The cell cycle distribution and apeptosis were detected by flow cytometic analysis. In addition, apeptosis was detected by Hoechst3358/PI fluorescent staining and the expression of Bcl-2 and Bax protein were detected by using western blot assay. Results： Cureumin inhibited the proliferation of HeLa cells in a does-and time-dependent manner. The cell growth was arrested at S stage. And aneuploid peak was observed by FCM. Fluorescent staining can detect the apoptotic cells. The expression of Bax was increased and the Bcl-2 was unchanged. Conclusion： Curcumin not only inhibits proferation but also induces apeptosis of HeLa cells. The possible mechanism is related with the increasing of Bax.