Study of Sonic hedgehog signaling pathway related molecules in gastric carcinoma

AIM： To study the expression of Sonic hedgehog pathway-related molecules, Sonic hedgehog （Shh） and Glil in gastric carcinoma. METHODS： Expression of Shh in 56 gastric specimens including non-cancerous gastric tissues, gastric adenocarcinoma, gastric squamous cell carcinoma was detected by RT-PCR, in situ hybridization and immunohistochemistry. Expression of Glil was observed by in situ hybridization. RESULTS： The positive rate of Shh and Glil expression was 0.0%, 0.0% in non-cancerous gastric tissues while it was 66.7%, 57.8% respectively in gastric adenocarcinoma, and 100%, 100% respectively in gastric squamous cell carcinoma. There was a significant difference between the non-cancerous gastric tissues and gastric carcinoma （P 〈 0.05）. Elevated expression of Shh and Glil in gastric tubular adenocarcinoma was associated with poorly differentiated tumors while the expression was absent in gastric mucinous adenocarcinoma. CONCLUSION： The elevated expression of Shh and Glil in gastric adenocarcinoma and gastric squamous cell carcinoma shows the involvement of activated Shh signaling in the cellular proliferation of gastric carcinogenesis. It suggests Shh signaling gene may be a new and good target gene for gastric tumor diagnosis and therapy.

瞄准：学习声音的刺猬的表示小径相关的分子，声音的刺猬(嘘) 和在胃的癌的 Gli1。方法：在包括非癌的胃的纸巾的 56 个胃的标本的嘘的表示，胃的腺癌，胃的有鳞的房间癌被 RT-PCR，原位杂交和免疫组织化学检测。Gli1 的表示被原位杂交观察。结果：嘘和 Gli1 表示的积极的率是 0.0% ， 0.0% 在非癌的胃的纸巾当它是 66.7% 时， 57.8% 分别地在胃的腺癌，和 100% ， 100% 分别地在里面胃的有鳞的房间癌。在非癌的胃的纸巾和胃的癌(P<0.05 ) 之间有有效差量。当表示在胃的粘蛋白的腺癌是不在的时，在胃的管状的腺癌的嘘和 Gli1 的提高的表示与糟糕区分的肿瘤被联系。结论：在胃的腺癌和胃的有鳞的房间癌的嘘和 Gli1 的提高的表示显示出在胃的致癌作用的细胞的增长发信号的激活的嘘的参与。它建议表明基因的嘘可以是为胃的肿瘤诊断和治疗的新、好的目标基因。