Effects of adenoviral-mediated gene transduction of NK4 on proliferation, movement, and invasion of human colonic LS174T cancer cells in vitro

瞄准：为了调查 recombinant 侵入人体气管粘膜的病菌向量的禁止的效果，那表示 NK4，人的 hepatocyte 生长因素(HGF ) 的一种截断的形式，在人的结肠的腺癌房间上为未来 NK4 基因癌症建立一个基础的在试管内治疗。方法：从 LS174T 人的结肠的腺癌房间线的房间在肿瘤房间增长， scatter，移植，和地下室膜侵略上感染 recombinant 侵入人体气管粘膜的病菌 rvAdCMV/NK4 和操作的效果被估计。感染表示贝它牛乳糖的 recombinant 侵入人体气管粘膜的病菌向量(Ad-LacZ ) 的房间用作控制。结果：我们发现那个 rvAdCMV/NK4 表达式稀释了导致 HGF 的肿瘤房间 scatter，移植，和地下室膜侵略(P【0.05 ) ，但是没禁止肿瘤房间增长。结论：导致 HGF 的 LS174T 肿瘤房间 scatter，移植，和侵略能被 recombinant 表示 NK4 侵入人体气管粘膜的病菌反对。

AIM： To investigate the inhibitory effects of a recombinant adenovirus vector that expresses NK4, a truncated form of human hepatocyte growth factor （HGF）, on human colonic adenocarcinoma cells in vitro to establish a basis for future NK4 gene cancer therapy. METHODS： Cells from the LS174T human colonic adenocarcinoma cell line were infected with recombinant adenovirus rvAdCMV/NK4 and the effects of the manipulation on tumor cell proliferation, scatter, migration, and basement membrane invasion were assessed. Cells infected with a recombinant adenovirus vector （Ad-LacZ） expressing β-galactosidase served as the controls. RESULTS： We found that rvAdCMV/NK4 expression attenuated HGF-induced tumor cell scatter, migration, and basement membrane invasion （P 〈 0.05）, but did not inhibit tumor cell proliferation. CONCLUSION： HGF-induced LS174T tumor cell scatter, migration, and invasion can be antagonized by the recombinant NK4-expressing adenovirus.