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Preparation, Characterization, and Application of Antiharpin_(xoo) Antibody 被引量：2

Preparation, Characterization, and Application of Antiharpin_(xoo) Antibody
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摘要 Polyclonal antiharpinxoo rabbit antibody has been prepared successfully using purified harpinxoo protein as an immunogen. The ELISA titer of the antiserum against harpinxoo was about 1：2 000. Western blot analysis showed that the antiserum could bind to the expression harpinxoo protein in particular, hrfl, encoding harpinxoo, is an expression in transgenic rice, detected by antiharpinxoo rabbit antibody. The rabbit antibody against harpinxoo can be used to study further about the biological function, harpinxoo localization, and hrfl gene expression in other plants. Polyclonal antiharpinxoo rabbit antibody has been prepared successfully using purified harpinxoo protein as an immunogen. The ELISA titer of the antiserum against harpinxoo was about 1：2 000. Western blot analysis showed that the antiserum could bind to the expression harpinxoo protein in particular, hrfl, encoding harpinxoo, is an expression in transgenic rice, detected by antiharpinxoo rabbit antibody. The rabbit antibody against harpinxoo can be used to study further about the biological function, harpinxoo localization, and hrfl gene expression in other plants.
作者 SHAO Mini LI Ming PAN Xiao-mei WANG Jin-sheng
机构地区 Key Laboratory of Monitoring and Management of Plant Diseases and Insects
出处 《Agricultural Sciences in China》 CAS CSCD 2006年第7期512-516,共5页 中国农业科学（英文版）
关键词 harpinxoo polyclonal antibody Western blot expression analysis in transgenic plants harpinxoo, polyclonal antibody, Western blot, expression analysis in transgenic plants
分类号 S4 [农业科学—植物保护]
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  • 1邵敏,王金生.转hrfA_(Xoo)基因水稻对白叶枯病的抗性[J].南京农业大学学报,2004,27(4):36-40. 被引量：22
  • 2王冬生,匡开源,李琳一,於文俊,袁永达,谢显传,瞿培荣,程银坤,王次马.设施栽培番茄软腐病的鉴别、发生和防治研究[J].上海农业学报,2006,22(2):5-8. 被引量：4
  • 3李小娟,刘二明,肖启明,苏世杰,赵志祥.稻曲病研究进展[J].农药研究与应用,2006,10(3):9-12. 被引量：29
  • 4顾林,文良柱,吴国球,徐寒梅,沈子龙.Thanatin突变体在大肠杆菌中的表达及纯化[J].东南大学学报（医学版）,2007,26(3):220-224. 被引量：4
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Agricultural Sciences in China
2006年 第7期

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