中国恶性疟原虫MSP1第13～17区基因和MSP2基因的研究

StudyongenesMSP2andregion13～17inMSP1fromtwochineseisolatesofplasmodiumfalci┐parum

恶性疟原虫裂殖子表面蛋白１（ＭＳＰ１）和２（ＭＳＰ２）是恶性疟原虫无性红内期重要的候选疫苗组分。为设计制作安全有效的恶性疟疫苗提供理论依据，根据文献报道的恶性疟原虫ＭＡＤ２０ＭＳＰ１和ＦＣ２７ＭＳＰ２ＤＮＡ序列设计并合成两对引物，对中国恶性疟原虫云南省勐腊县勐罕分离株ＣＭＨ１／ＹＮ和海南省昌江县ＦＣＣ１／ＨＮ分离株ＭＳＰ１第１３～１７区基因和ＭＳＰ２基因进行聚合酶链反应（ＰＣＲ）扩增及鉴定。ＰＣＲ产物进一步根据含单一酶切位点ＥｃｏＲＩ和ＸｂａＩ进行酶切鉴定，确证为目的基因，扩增序列分别为１．７Ｋｂ和０．８Ｋｂ。结果发现中国恶性疟原虫ＣＭＨ１／ＹＮ和ＦＣＣ１／ＨＮ分离株ＭＳＰ１第１３～１７区基因和ＭＳＰ２基因与巴布亚－新几内亚恶性疟原虫株ＭＡＤ２０和ＦＣ２７在这个区域内的基因一致，表现出高度同源性。这一发现为开发预防恶性疟的疫苗和药物奠定了基础。

Themajormerozoitesurfaceprotein-l(MSP1)and-2(MSP2)aretwoimportantvaccinecandidatesforahumanasexualbloodstageofPlasmodiumfalciparum.Inordertomakesafeandefectivevaccinesoffalciparummalarisandprovideatheoreticalbasis,genomicDNAsamplesoftwoPlasmodiumfalciparumisolateswerecolectedfromfalciparummalariapatientsinMenglaCounty,YunnanProvince(CMH1/YN)andinChangjiangCounty,HainanProvince(FCC1/HN).Thesampleswereusedasthetemplatesforpolymerasechainreaction(PCR)amplificationandthetwopairsofoligonucleotidesfromthegenesofFC27MSP2andtheregion13～17inMAD20MSP1ofPapuaNewGuineastrainofPlasmodiumfalciparumwereusedasprimers.ThePCRproductswereverifedbydigestionofuniqueEcoRIandXbaI,andthegeneratingfragmentswereidenticalwiththespecificgenes1.7Kband0.8Kbwhichweredesignedbyourselves.OurfindingsindicatethatbothChineseisolatesCMH1/YNandFCC1/HNofPlasmodiumfalciparumexaminedcontainedgenessimilartothosedefinedinknownMAD20MSP1andFC27MSP2alelicdimorphicgroup.Ithasbeensuggestedbytheexperimentalstudiesthattheremaybegoodprospectfordevelopingvac-cinesoffalciparummalariaanddrug,anditisworthyoffurtherinvestigation.