摘要
为了探讨丙型肝炎病毒（ＨＣＶ）母婴传播是否发生于产前，用逆转录－套式－聚合酶链反应检测孕妇外周血及其引产胎肝匀浆中ＨＣＶＲＮＡ，同时用原位杂交技术检测引产胎肝细胞中ＨＣＶＲＮＡ。结果从６例外周血ＨＣＶＲＮＡ阳性的中期妊娠孕妇的引产胎肝组织匀浆中，４例胎肝检出ＨＣＶＲＮＡ，其中２例胎肝组织切片中发现ＨＣＶＲＮＡ原位杂交阳性胎肝细胞。阳性着色局限于胎肝细胞的胞浆内。提示ＨＣＶ母婴传播可发生于产前妊娠期，ＨＣＶＲＮＡ可定位于胎肝细胞浆内。
Inordertorequireintowhethermother-to-infanttransmissiontakesplaceprenatalyornot,theperipheralserafrompregnantwomenandtheirabortedfetalliverhomogenateswerede-tectedforHCVRNAwithreversetranscription-nestedpolymerasechainreaction(RT-Nested-PCR),andtheabortedfetalhepatocyteswereprobedforHCVRNAwithinsituhybridizationaswel.TheresultsshowedthatHCVRNAwasdetectedin4of6fetalliverhomogenatesdeducedfromtheseralHCVRNA-positivewomenwhowerebeyondmiddlestageofpregnancy.Amongthe4fetallivertissues,2wereHCVRNApositivewithinthefetalhepatocytesbyinsituhybridization.Thepositivestaininglocatedlimitedlywithinthefetalhepaticcytoplasm.Theseresultsindicatetheexistenceofprenatalmother-to-infanttransmissionofHCVandthelocationofHCVRNAwithinthefetalhepaticcytoplasm.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1996年第4期218-221,共4页
Chinese Journal of Infectious Diseases
基金
国家自然科学基金
关键词
丙型肝炎病毒
母婴传播
胎肝细胞
原位杂交
HCVMother-to-infanttransmissionFetalhepatocyteInsituhybridization
