高效液相色谱法测定动物组织中四环素族抗生素残留

Determination of Tetracyclines Residue in Animal Tissues by High Performance Liquid Chromatography

建立了一种使用高效液相色谱同时测定动物组织中土霉素(OTC)、四环素(TC)和金霉素(CTC)残留的方法。样品的预处理采用5%高氯酸溶液去除样品中蛋白质。采用Kromasil C18柱(250×4.6mm,5μm);0.01mol/L NaH2PO4.2H2O(pH=3)—60/40的乙腈/水(v/v)为流动相;流速1.0mL/min;检测波长为355nm;梯度洗脱。四环素族抗生素(TCs)的回收率在89.4%～101.2%之间,相对标准偏差RSD在1.41～3.50%。本方法可靠、灵敏、经济、快速。

The residual of oxytetracycline（OTC）, tetracycline（TC）, chlortetracycline（CTC） in animal tissues could be separated and determined by high performance liquid chromatography, after deproteinized by 5% HClO4. A Kromasil C18 column （250 × 4.6mm, 5 μ m） was used; 0.01mol/L NaH2PO4 · 2H2O（pH=3）-60/40 acetonitrile-water was used as a mobile phase; The flow rate of the mobile phase was kept at 1.0mL/min; The residues using gradient elution were monitored by UV detector at 355mn. The recoveries of tetracyclines were 89.4-101.2%, and the relative standard were less than 3.50%.This method was reliable, sensitive, economical and rapid.