摘要
采用摇瓶培养法对牛舌菌液体发酵的条件进行了研究。通过L9(34)正交试验确定的牛舌菌液体发酵的最适培养基组成为:玉米粉30.0g,黄豆粉7.0g,KH2PO42.0g,MgSO4·7H2O1.0g,VB10.01g;该菌株液体发酵的优化条件为:初始pH4.6～5.4,培养温度28℃,装液量60%,振荡速度210r/min,发酵时间6d,菌丝体生物量达14.7241g/50ml。
This paper explore that suitable fermentative conditions, which affect the mycelia growth of Fismlina hepatica. Experimental results indicated that the optimum composition of a fermentative medium is as following： corn powder 30.0g, soya powder 7.0g, KH2PO4 2.0g, MgSO4 · 7H2O 1.0g, VB1 0.01g; pH4.6～5.4, the shaker rotation speed is at 210r/min, the temperature is at 28℃, the optimal culture media capacity is 60%. The highest yield of mycelia was obtained after fermentation for 6 days, the weight of mycelia reached 14.7241g/50ml fermentation liquid.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第7期173-176,共4页
Food Science
基金
陕西省教育厅专项科研基金项目(99JK109)
关键词
牛舌菌
液体发酵
菌丝体
Fistulina hepatica
liquid fermentation
mycelia
