亚花松萝多糖的提取与分离工艺的优化

Optimal Design of Extraction and Separation Procedure for the Usnea subfloridana Polysaccharide

本文研究亚花松萝多糖的提取与分离工艺的优化。亚花松萝多糖提取工艺各因素的最佳水平为热水体积200ml(每4g松萝)、70～80℃提取三次,每次2h。其中对亚花松萝多糖含量影响最显著的因素是提取次数,然而提取温度在一定范围内无显著影响。乙醇沉淀多糖的最佳浓度为80%一倍,Sevage试剂除游离蛋白时的体积比为3:1,反复脱蛋白五次,每次振荡30min。多糖的最佳得率为6.86%,总糖含量为76%。

This paper studied the optimum preparation procedure for extraction and septeration of the Usnea subfloridana polysaccharide. The optimum extraction conditions of Subfloridana polysaccharide are： water 200ml, Usnea subfloridana 4g, extracting-temperature 70～80℃, repeated-times 3 and extracting-time 2h. The results showed that the variance of the factor reapeated-times assumes statistical significance, whereas the extracting-temperature is not statistically significant. A one-factor test results indicated that the optimum conditions of precipitating polysaccharide with 70% alcohol, and removing proteins with sevage agent to gain gratifying effictiveness are as follows： volume ratio 2：1, oscillation-time 30min and repeated times 7 with polysaccharide yield 6.86% and total sugar content 76%.