海洛因依赖患者外周血清可溶性Fas及Fas配体的变化

The Changes of Peripheral Serum sFas and FasL in Heroin Addicts

目的:探讨海洛因依赖患者血清可溶性Fa(ssFas)和Fas配体(FasL)水平的变化及其意义。方法:采用双抗体夹心酶联免疫吸附法(ELISA),检测46例海洛因依赖患者血清中sFas和FasL水平,与正常人群作对照研究,并比较不同方式依赖者的sFas和FasL变化。结果:海洛因依赖者血清中sFas水平与对照组比较无统计学差异,而FasL水平明显高于对照组,差异具有统计学意义(P<0.01)。24例注射依赖者血清FasL水平较对照组高,差异具有统计学意义(P<0.05)。结论:海洛因依赖者血中FasL水平异常,提示海洛因滥用增加了Fas介导的细胞凋亡,且与用毒的方式有一定关系。这可能是海洛因依赖者免疫功能低下的重要原因之一。

Objective： To investigate the changes of soluble Fas （sFas） and Fas Ligand （FasL） levels in peripheral serum of heroin addicts. Methods： Concentrations of sFas and FasL were measured by a dual antibody sandwich enzyme-linked immunosorbent assay （ELISA） in 46 heroin addicts as well as 39 normal controls. The concentrations were compared with the normal control and compared between the groups with different abusing route. Results： The serum FasL levels of the heroin addicts were significantly higher than that of the controls （P 〈 0.01）. There was no significant difference of the serum sFas levels between heroin addicts and controls. The serum FasL levels were significantly higher in groups with intravenous mixed with intramuscular abusing routes than those of the controls （P 〈 0.05）. Conclusion： The results indicated that there was an abnormal serum levels of FasL in heroin addicts. It is suggested that heroin produce an increase in the Fas-mediated apoptosis and it might explain the decreased immunity function in heroin addicts.