复方中药安胃汤提高大鼠胃溃疡愈合质量的机制

Effect of Anweitang on gastric ulcer and it mechanism in experimental rats

目的:通过实验性乙酸大鼠胃溃疡模型,研究复方中药安胃汤提高慢性胃溃疡愈合质量机制．方法:40只Wistar大鼠随机平均分为正常对照组、模型组、安胃汤组和雷尼替丁．采用乙酸浸渍法建立胃溃疡模型,造模3 d后前两组分别用生理盐水灌胃,后两组分别用安胃汤和雷尼替丁灌胃．采用放射免疫方法和免疫组织化学方法观察复方中药安胃汤对大鼠胃溃疡模型愈合时血清表皮生长因子(EGF)和胃黏膜 EGF,表皮生长因子受体(EGFR)及转化生长因子-β1(TGF-β1)表达的影响．结果:模型组相比,安胃汤组和雷尼替丁组可提高血清EGF(1．12±0．24,0．99±0．15μg／L vs0．52±0．13μg／L,P<0．01),安胃汤组与雷尼替丁组相比差异也有显著意义(P<0．05)．与模型组相比,安胃汤组和雷尼替丁组可显著增强胃黏膜EGF、EGFR及TGF-β1表达(EGF: 29．7％±1．9％,26．5％±1．6％vs18．4％±2．0％, P<0．01:EGFR:29．6％±2．6％,25．9％±1．0％vs 20．4％±1．8％,P<0．01;TGF-β1:67．0％±2．0％, 49．5％±1．1％vs27．3％±1．0％,P<0．01),安胃汤组强于雷尼替丁组(均P<0．01)．结论:安胃汤可能通过提高血清EGF和增强胃黏膜EGF,EGFR及TGF-β1表达,增强胃黏膜保护作用而提高溃疡愈合质量．

AIM： To investigate the mechanism of Anweitang in the improvement of gastric ulcer healing quality. METHODS： Forty Wistar rats were randomly and averagely divided into group A, B, C and D. Acetic acid was used to establish the model of gastric ulcer in the rats of group B, C and D. The rats in group A and B were treated （ig） with normal saline while those in group C and D were treated （ig） with Anweitang and Ranitidine, respectively. Radioimmunoassay was used to determine the expression of serum epidermal growth factor （EGF）, and immunohistochemistry was applied to detect the expression of EGF, EGF receptor, and transforming growth factor-β1 （TGF-β1） in the gastric mucosa. RESULTS： In comparison with that in the group B, the expression of serum EGF was evidently enhanced in group C and D （1.12 ± 0.24, 0.99 ± 0.15 μg/L vs 0.52 ± 0.13 μg/L, P 〈 0.01）, and serum EGF expression was also significantly different between group C and D （P 〈 0.01）. Furthermore, the expression of mucosal EGF, EGFR and TGF-β1 were markedly higher in group C and D than those in group B （EGF： 29.7% ± 1.9%, 26.5% ± 1.6% vs 18.4% ± 2.0%, P 〈 0.01; EGFR： 29.6% ± 2.6%, 25.9% ＋ 1.0% vs 20.4% ± 1.8%, P 〈 0.01; TGF-β1： 67.0% ± 2.0%, 49.5% ± 1.1% vs 27.3% ± 1.0%, P 〈 0.01）. There were also significant differences between group C and D in the expression of mucosal EGF, EGFR and TGF-β1（all P 〈 0.01）. CONCLUSION： Anweitang may improve the healing quality of gastric ulcer by enhancing the expression serum EGF and mucosal EGF, EGFR, and TGF-β1.