玻璃体切除标本和膜剥离标本的免疫组织化学对比研究

Immunohistochemical Contrary Study of Vitrectomy Preparations and Membrane-peeling Specimens

用免疫组织化学方法检查了增殖性眼内疾病的16例玻璃体切除标本和11例视网膜前膜标本中人类主要组织相容性抗原Ⅱ类抗原(HLA-DR)和巨噬细胞抗原(CD68),以研究玻璃体切除标本在免疫组织化学检查中的意义。结果玻璃体切除标本 HLA-DR 阳性10例(62.5%),CD68阳性8例(50%)。可区别典型的膜结构12例(75%),4例标本仅见单个细胞、组织碎片和色素颗粒。同一患者两种收集标本方法对比共7例,玻璃体切除标本 CD68阴性5例,HLA-DR 阴性1例;而膜剥离标本在 PVR、外伤性 PVR 和牵引性视网膜脱离二者均为阳性。提示玻璃体切除标本收集简单容易,可用于增殖性眼内疾病的免疫组织化学研究。

Sixteen specimens obtained from vitreous deposit and eleven specimens obtained from epiretinal mem- branes of proliferative intraocular disorders(PID) were immunohistochemically investigated for human leuco- cyte antigen Ⅱ antigen (HLA-DR) and macrophage antigen (CD68) to determine the role of the vitreous preparation in immunohistochemistry.The results showed that 10 anti-HLA-DR(62.5%) and 8 anti-CD68 (50%) were found positive in the specimens of the vitreous deposit.Typical membrane structures were exist- ed in twelve samples (75%) from preparation of vitrectomy.In contrast with the results of two specimen-col- lecting methods for seven cases,1 HLA-DR and 5 CD68 were negative respectively in the preparation of vit- rectomy.However,both markers were found positive in all membrane-peeling specimens which were derived from the patients with proliferative vitreoretinopathy (PVR),traumatic PVR and traction retinal detachment. Conclusion:Collecting vitrectomy specimens was easier and simpler than epiretinal membrane and immuno- histochemistry was a reliable method for vitreous preparation in PID.