大熊猫DNA指纹在野生种群数量调查中的应用

DNA FINGERPRINTING APPLIED TO THE INVESTIGATION OF WILD POPULATION OF GIANT PANDA

本文用荧光素标记ＬＺＦ－１基因指纹探针，以四川省冕宁县冶勒野外大熊猫的脱落被毛及尽量新鲜的粪便作样品，进行了ＤＮＡ指纹检测。１．在相同或不同时间、巢域采集的粪便与被毛样品，显现出相同或不同的ＤＮＡ指纹图谱，达到个体认定的目的．表明了大熊猫野外脱落被毛和粪便，能作为ＤＮＡ指纹分析材料，进行野生种群数量调查．２．根据检测６个被毛和９个粪便样品的结果，认定该生境中有７只大熊猫个体，纠正了有８只和８±２只的记载．３．应用ＤＮＡ指纹技术及微机个体识别进行大熊猫野外数量调查，准确可靠，节省人力、物力和财力，能获得大熊猫在野外的真实个体数量。

Detecting the DNA fingerprints of hairs and excrements collected from wild the clear and high distinctive patterns of giant pandas living in Yele of Mianning County of Sichuan Province were obtained, and the results are as follows.1.The DNA fingerprints of hairs and excrements collected in different time and the same home range of the same giant panda were identical. It showed that detecting the hair and excrement DNA fingerprints can achieve the goal of identifying individuals of giant pandas living in wild.2. There are 7 giant pandas in the wild population of Yele of Mianning .County by detecting the DNA fingerprints of 15 samples including 6 hairs and 9 excrements, and it corrected the mistakes of having the numbers of 8 or 8±2 in the wild population of Yele.3. The method of DNA fingerprinting labelling oligonucleotide probe LZF-I with fluorescein applied to the investigation of wild population of giant panda is fast, accurate and economical, and it can obtain the true number of wild population.