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LHRH-PE40对胃癌细胞增殖抑制和诱导凋亡的实验研究 被引量：3

LHRH-PE40 inhibits proliferation and induces apoptosis in human gastric carcinoma cell
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摘要 目的观察新型免疫毒素LHRH-PE40对胃癌细胞的抑制作用并探讨其作用机制。方法将体外培养的胃癌细胞分别加入不同浓度的LHRH-PE40进行干预后,测定肿瘤细胞生长情况的变化,并应用流式细胞仪检测LHRH-PE40诱导肿瘤细胞凋亡情况。结果免疫毒素LHRH-PE40对人胃癌细胞系BGC-823的增殖有明显的抑制作用,同时促进了细胞的凋亡。结论免疫毒素LHRH-PE40对胃癌细胞的增殖状态有明显的抑制作用。 Objective To investigate the inhibition effect of immunotoxins LHRH-PE40 on the gastric carcinoma and its action mechanism. Methods The gastric cancer cell lines cultured in vitro were interfered with different concentrations of LHRH-PE40 to detect the growth of carcinoma cell to determine by MTT assay whether LHRH-PE40 induce carcinoma cell apoptosis. Results LHRH-PE40 significantly inhibited the proliferation and induced the apoptosis of gastric carcinoma cell line BGC-823. Conclusions LHRH-PE40 has obvious inhibition effect on the proliferation of gastric cell lines.
作者 宋燕 王凌燕 姜洋 高宇飞 吴广谋 朱平
机构地区 吉林大学中日联谊医院 解放军军事医学科学院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2006年第7期928-929,共2页 Chinese Journal of Gerontology
基金 国家重点科技攻关项目(96-901-05-101) 专利申请号(9912205.9)
关键词 LHRH-PE40 胃癌 凋亡 LHRH-PF40 Gastric carcinoma Apoptosis
分类号 R735.2 [医药卫生—肿瘤]
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中国老年学杂志
2006年 第7期

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