酶联免疫印迹技术分析广州管圆线虫雌虫抗原

Analysis of Antigens of Angiogtrongylus cantonensis Female Worms by ELIB

应用酶联免疫印迹技术（ＥＬＩＢ）对广州管圆线虫雌成虫粗抗原（Ａｇ－Ｆ）蛋白组分及其免疫学特性进行分析。ＳＤＳ－ＰＡＧＥ显示，Ａｇ－Ｆ至少有３０条多肽区带，分子量分别为１１０、９５、７９、６８、６０、４４、４０、２９、２３、２１和１８ｋＤ等蛋白组分被实验感染广州管圆线虫大鼠血清识别，提示Ａｇ－Ｆ这些组分具有诊断价值。４２ｋＤ组分蛋白被猪蛔虫抗原免疫大鼠血清识别。实验感染广州管圆线虫大鼠血清抗Ａｇ－Ｆ的１００、９８、７９、６８和４４ｋＤ等组分的抗体出现于感染后第７ｄ。

Female worm antigens (Ag-F) of Angiostrongylus cantonensis(A. c. )were analysed by SDS-PAGE and ELIB, the results revealed that the SDS-PAGE patterns of Ag-F consisted of at least 30 polypeptide bands. Applying ELIB, the results showed that 11 bands of polypeptides could be recognized at least by sera from rats infected with A. c.; a band could be recognized by sera from rats immunized with Ascaris suum antigen; but this antigen had no cross-reactions with the sera from rats infected with S. japonicum or immunized with sparganum antigen of S. mansoni. The Ag-F evidently evoked the antibody-producing response in the experimental rat as early as 7 days after infection.