摘要
为了探讨胃癌病因，我们用聚合酶链式反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）技术分析了胃癌高发区胃癌组织Ｐ５３基因的改变。９０例胃癌中有４５例Ｐ５３基因发生改变，其中３９例为点突变，６例为缺失和插入突变，Ｐ５３基因点突变以Ｇ→Ａ和Ａ→Ｇ突变为主（６６．７％），Ｇ→Ｔ和Ｔ→Ｇ占１５．５％，这些结果提示Ｐ５３基因改变与胃癌的发生有密切关系。
Tostudyetiologyofgastriccancer,polymerasechainreaction-singlesequenceconfig-urationpolymorphism(PCR-SSCP)methodwasusedtoanalyzethechangesofP53geneingastriccancertissuesderivedfromareaswithhighprevalenceofit.ChangesinP53geneoccurredsin45of90specimensofgastriccancertissues,and39ofthemshowedfocalmutationandsixweredeletionandinsertion.Amongthepointmutation,66.7%weresubstitutionofAtoG(G-A)orGtoA(A-G),andonly15.5%weresubstitutionofTtoG(G-T)orGtoT(T-G).TheseresultssuggestthatthereisacloserelationshipbetweenP53genemutationandoccurrenceofgastriccancer.Basedonthecharacteristicsofbasepairmutation,itispostulatedthatmethylatingcarcinogenandpolycyclicaromatichydrocarbonfromenvironmentcontributedtotheaboveresults.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1996年第6期343-346,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金
