口腔黏膜癌变过程中Slit蛋白的表达及其与血管生成的关系

Relationship between Slit expression and angiogenesis in carcinogenesis of human oral mucosa

目的:研究Slit蛋白在口腔黏膜癌变过程中的表达及其与肿瘤血管形成的关系。方法:采用免疫组织化学Envision法检测40例人口腔鳞状细胞癌、18例上皮单纯增生、20例上皮异常增生、20例原位癌及19例正常口腔黏膜标本Slit、VEGF的表达和MVD。结果:口腔鳞癌组中有34例Slit表达阳性,与正常黏膜组及癌前病变组相比有显著性差异(P<0.01),而19例正常口腔黏膜仅有1例表达为弱阳性,与原位癌组及上皮异常增生组之间有显著性差异(P<0.01,P<0.05)。VEGF的表达趋势与Slit蛋白一致,Slit蛋白和VEGF表达均与MVD呈显著正相关(P<0.01)结论:口腔鳞癌癌变过程中Slit蛋白阳性表达与其恶性程度呈正相关,Slit在口腔鳞癌中有促血管形成的作用,并可能对口腔鳞癌的发展过程起重要作用。

bjective： To study the expression of Slit protein and vascular endothelial cell growth factor（VEGF） in carcinogenesis of human oral mucosa and to investigate the relationship between the Slit and angiogenesis. Methods： The expression of Slit protein and VEGF was detected using immunohistochemical method, microvessel density （MVD） was counted following immunostaining with anti-vWF antibody in 40 cases of human oral squamous cell carcinoma（ OSCC）, 18 cases of simple hyperplasia,20 cases of dysplasia,20 cases of carcinoma in situ and 19 cases of normal oral mucosa（NOM）. Results The positive expression of Slit was detected in 34 cases of OSCC,4 of simple hyperplasia,7 of dysplasia,9 of carcinoma in situ and 1 of NOM（ P 〈0. 01 ）. The trend of VEGF expression was accordant with Slit and significantly positive correlation was also found between the MVD and the expression of Slit and VEGF（ P 〈0. 01 or P 〈 0. 05）. Conclusion： Slit has a positive correlation with angiogenesis of OSCC, and may play a positive role in OSCC development.