两种冠修复贵金属合金与种植钛对龈下优势菌黏附的影响

The effect of two kinds of crown materials attached to implant Ti on the adhesion of subgingival predominant bacteria

目的:研究龈下优势菌在2种冠修复材料(金铂合金与金钯合金)与种植钛材料表面的黏附特征。方法:将金铂合金与金钯合金分别粘结在种植纯钛板上,与4种龈下优势菌:牙龈卟啉单胞菌(Pg),伴放线放线杆菌(Aa),中间普氏菌(Pi),具核梭杆菌(Fn)共同厌氧孵育。采用菌落形成单位(CFU)计数法量化测定培养试件表面的细菌黏附量,扫描电镜观察试件表面细菌黏附的情况。结果:Aa在2组试件上的黏附量最多,其次为Fn、Pg,最少的为Pi;龈下优势菌在金铂合金-钛试件表面细菌黏附量明显多于金钯合金-钛试件。结论:①4种龈下优势菌对同一合金试件的附着能力有差异:Aa>Fn>Pg>Pi。②金钯合金对细菌的生长与黏附有抑制作用。

Objective：To study the effect of Au-Pt and Au-Pd attached to Ti implant on the adhesion of different subgingival predominant bacteria. Methods：Two kinds of crown materials（ Au-Pt and Au-Pd）were attached to Ti implant respectively. Four subgingival predominant anaerobical bacteria Actinobacillus actinomycetemcomitans （ Aa ） , Porphyromanus gingivalis （Pg） , Fusobacterium nucleatum （ Fn ） , and Prevotella intermedia （Pi） were cultured on the samples of the materials respectively, the adhesion of the bacteria on the materials was investigated by means of clone forming unit （CFU） method. The morphology of the bacteria adhered to the materials was examined by scanning electron microscopy （SEM）. Results：The adhesion（×10^5 CFU/ml） of Aa,Pg,Pi and Fn on Au-Pt was 9.14±0. 85,2.87±0. 18,0.77±0. 07 and 3.03±0. 81, that on Au-Pd 1.68±0. 18,0.83 ±0. 08,0. 17±0. 04 and 0. 83 ±0. 13, respectively. The adhered bacteria of the 4 kinds on the surface of Au-Pt alloy samples were more than those on Au-Pd （ P 〈0. 01 ）. SEM observation showed samillar results. Conclusion： There is a difference in the adhereing ability of the four subgingival predominant bacteria：Aa 〉 Fn 〉 Pg 〉 Pi. Au-Pt alloy does not resist the growth and adhesion of the bacteria, but Au-Pd does.