类胰岛素生长因子对人肺成纤维细胞糖代谢及功能的影响
被引量：1
摘要
研究类胰岛素生长因子（IGF-1）对肺成纤维细胞（LF）糖代谢及其分泌细胞外基质蛋白的调控作用，为从LF环节干预肺纤维化提供线索。
材料与方法正常人肺LF株接种于75cm×75cm培养瓶中，用含10％胎牛血清、100U青霉素＋链霉素（100μg／m1）的DMEM培养液中37℃孵育。选择长成良好的单层但尚未融合的细胞分组：（1）对照组；（2）IGF-1100ng／ml组；（3）IGF-1200ng／ml组；（4）IGF-1300ng／ml组。
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2006年第7期490-491,共2页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家自然科学基金资助项目(30170408)
参考文献5
-
1Allen CB, Guo XL, White CW. Changes in pulmonary expression of hexokinase and glucose transporter mRNA in rats adapts to hyperoxia. Am J Physiol, 1998,274(3 Pt 1 ) :L320-L329.
-
2Yateman ME, Claffey DC, Cwyan Hughes SC , et al. Cytokines modulate the sensitivity of human fibroblasts to stimulation with insulin-like growth factor-1 ( IGF-1 ) by altering endogenous IGF-binding protein production. J Endocrinol, 1993,137 : 151-159.
-
3Pala L, Giannini S, Rosi E, et al. Direct measurement of IGF-Ⅰ and IGFBP-3 in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis. J Endocrinol Invest ,2001,24:856-864.
-
4Wynes MW, Riches DW. Transcription of macrophage IGF-Ⅰ exon-1 is positively regulated by the 5 '-untranslated region and negatively regulated by the 5 ' -flanking region. Am J Physiol Lung Cell Mol Physiol, 2005,288 : L1089 -L1098.
-
5Krein PM, Sabatini PJ, Tinmouth W, et al. Localization of insulin-like growth factor-I in lung tissues of patients with fibroproliferative acute respiratory distress syndrome. Am J Respir Crit Care Med ,2003,167 :83-90.
同被引文献24
-
1Badid C, Vincent M, Fouque D, et al. Myofibroblast : a prognostic marker and target cell in progressive renal disease. Ren Fail, 2001,23 : 543-549.
-
2Tomasek JJ, Gabbiani G, Hinz B, et al. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol, 2002,3 : 349-363.
-
3Hinz B, Gabbiani G. Mechanisms of force generation and transmission by myofibroblasts. Curr Opin Biotechnol, 2003,14:538-546.
-
4Zhang K, Rekhter MD, Gordon D, et al. Myofib.roblasts and their role in lung collagen gene expression during pulmonary fibrosis: a combined immunohisto-chemieal and in situ hybridizationstudy. Am J Pathol, 1994,145 : 114-125.
-
5Zhang H, Gharaee-Kermani M, Zhang K, et al. Lung fibroblast contractile and a-smooth muscle actin phenotypic aherative inbleomycin-induced pulmonary fibrosis. Am J Pathol, 1996, 148:527-537.
-
6Kuhn C, McDonald JA. The roles of the myofibroblast in idiopathic pulmonary fibrosis: uhrastructural and immunohistochemical features of sites of active extracellular atrix synthesis. Am J Pathol, 1991,138 : 1257-1265.
-
7Ramos C, Montano M, Garcin-Alrarez J, et al. Fibroblasts from idiopathic fibrosis and normal lungs differ in growth rate, apopsis,and tissue inhibitor of metalloproteinases expression. Am J Respir Cell Mol Biol, 2001,24 : 591-598.
-
8Wang R, Ramos C, Joshi I, et al. Human lung myofibroblast- derived inducers of alveolar epithelial apoptosis identified as angiotensin peptides. Am J Physiol, 1999,277 : L1158-L1164.
-
9Uhal BD, Joshi I, Hughes WF, et al. Alveolar epithelial cell death adjacent to underlying myofibroblasts in advanced fibrotic human lung. Am J Physiol, 1998,275 : L1192-L1199.
-
10Bradbury DA,Newton R,Zhu YM,et al. Effect of bradykinin, TGF-β1 ,IL-1β,and hypoxia on COX-2 expression in pulmonary artery smooth muscle cells. Am J physiol. Lung Cell Mol Physiol, 2002,283 : L717-725.
-
1柯希煌,练克俭,翟文亮.骨生长因子对骨形成的协同作用研究进展[J].中国矫形外科杂志,2005,13(20):1582-1583. 被引量：2
-
2高岩,邓洁英,黄秉仁.类胰岛素生长因子(一)[J].国外医学（内分泌学分册）,1992,12(4):186-190. 被引量：5
-
3邓小玲,许铭炎,李克,傅玉才.凝血酶诱导人肺成纤维细胞释放单核细胞趋化蛋白-1[J].汕头大学医学院学报,2010,23(1):10-12. 被引量：3
-
4张晓,周艳红,夏光.青少年神经性厌食患者治疗前后血清IGF-Ⅰ和瘦素浓度的变化[J].中国基层医药,2008,15(6):921-922.
-
5朱兰,边旭明,钱美伦,樊庆泊.类胰岛素生长因子与胎儿生长发育关系的研究[J].中国实用妇科与产科杂志,2001,17(9):539-541. 被引量：3
-
6糖尿病[J].国外科技资料目录（医药卫生）,1997(9):60-61.
-
7吴淑会,刘臣.胰岛素样生长因子Ⅰ(IGF-Ⅰ)与各疾病的相关性研究进展[J].中国实验诊断学,2014,18(7):1214-1216. 被引量：12
-
8陈光华.2型糖尿病患者血清IGF-2和SIL-2R水平检测的临床意义[J].放射免疫学杂志,2003,16(2):106-106. 被引量：4
-
9人体细胞学[J].国外科技资料目录（医药卫生）,1999(7):5-5.
-
10黄维丽,朱崇昭,卫江亮.54例糖尿病患者血清IGF-2测定的临床意义[J].放射免疫学杂志,2001,14(1):25-26. 被引量：1
