摘要
目的探讨维生素D受体(VDR)基因TaqⅠT/C和FokⅠC/T多态性是否与HBV感染结局之间存在关联。方法采用病例-对照研究方法,募集212例HBV自限性感染者、244例无症状HBsAg携带者和391例慢性乙型肝炎患者作为研究对象,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对VDR基因TaqⅠ和FokⅠ基因进行分型测定。结果慢性乙肝组VDR-FokⅠC等位基因频率为45.8%,显著高于自限性感染组的38.2%(X2=6.43,P=0.01)。慢性乙型肝炎组VDR-FokⅠ的TT、TC及CC基因型频率分别为30.7%、47.1%和22.2%,与自限性感染组的基因型频率41.0%、41.5%和17.5%相比差异有统计学意义(X2=6.76,P=0.03)。单因素分析显示,携带CC/CT基因型的个体感染HBV后,增加发生慢性乙型肝炎的风险(OR=1.57,P=0.01),经Logistic回归分析,控制性别的混杂作用后,携带FokⅠCC/CT基因型者发生慢性乙肝的风险是TT基因型携带者的1.7倍(OR=1.70,P=0.021)。未发现VDR-TaqⅠT/C多态性与HBV感染结局存在显著关联。单体型分析显示,TaqⅠT-Fok C在慢性乙型肝炎组的频率为2.3080%,显著高于HBV自限性感染组的0.5391%(X2=6.08,P=0.01);TaqⅠT-FokⅠT在慢性乙型肝炎组的频率为1.5283%,显著低于HBV自限性感染组的3.7061%(X2=5.65,P=0.02)和显著低于HBV携带组的3.4820%(X2=5.12,P=0.02)。结论VDR基因多态性可能是影响HBV感染结局的因素之一。
Objective To determine whether TaqⅠ T/C and FokⅠ C/T polymorphisms of Vitamin D Receptor (VDR) gene was associated with the outcomes of hepatitis B virus (HBV) infection. Methods A total of 212 HBV self-limited infection individuals, 244 asymptomatic HBsAg carriers and 391 chronic hepatitis B (HB) patients were recruited to conduct a case-control study. VDR-TaqⅠ T/C and VDR-FokⅠ C/T polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The frequency of VDR-FokⅠ allele C in the chronic HB patients was 45. 8%, significantly higher than 38.2% of the self-limited infection individuals ( X^2 = 6.43, P = 0.01 ). The frequencies of VDR-FokⅠ genotypes TF, TC, and CC in HB patients were 30. 7%, 47.1%, and 22. 2% respectively, and 41.0% ( TT), 41.5 % ( TC), and 17.5 % (CC) in the self-limited infection individuals. There was a statistically significant difference between HB patients and self-limited infection individuals (X^2 = 6. 76,P = 0. 03). The results of univariate analysis showed that the subjects canting VDR-FokⅠ CC / TC genotype had 1.57-fold elevated risk for developing chronic HB when they were infected HBV ( OR = 1.57 ,P =0. 01 ). A multiple logistic regression analysis revealed that VDR-FokⅠ CC/CT was independently associated with chronic HB after adjusting both potential confounding effects of gender ( OR = 1.70, P =0. 021). The statistically significant association between Taq I T/C polymorphism and outcome of HBV infection was not demonstrated in the study. The frequency of haplotype TC of VDR-TaqⅠ and FokⅠ in HB patients was 2. 3080%, significantly higher than O. 5391% of the self-limited infection individuals ( X^2 = 6. 08, P =0. 01). However, the frequency of haplotype TT in the HB patients was 1. 5283%, significantly lower than 3. 7061% of the self-limited infection individuals ( X^2 = 5.65, P = 0. 02) and 3. 4820% of the HBV carriers (X^2 = 5. 12, P = 0. 02 ). Conclusion VDR gene polymorphism is probably an influence factor on the genetic susceptibilitv of HBV infaction.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第28期1952-1956,共5页
National Medical Journal of China
基金
北京市科委重大项目资助(H020920020590)
关键词
肝炎
乙型
受体
骨化三醇
多态性
单核苷酸
单元型
Hepatitis B
Receptors,calcitriol
Polymorphism,single nucleotide
Haplotypes