应用PCR技术检测肝内胆管结石中蛔虫ITS-2序列

Detection of ascarid ITS-2 sequence out of intrahepatic bile duct stones by PCR technique

目的通过分子生物学技术寻找原发性胆管结石内蛔虫脱氧核糖核酸存在的证据。方法从原发性胆管结石患者手术中获取的结石和肝胆管组织标本内分别提取结石和胆管壁组织脱氧核糖核酸,并对患者作蛔虫的粪检和血清学检查;针对蛔虫ITS-2序列设计引物,作聚合酶链反应。结果56例原发性胆管结石的脱氧核糖核酸中有12例扩增出蛔虫特异性的脱氧核糖核酸片段,经测序符合蛔虫脱氧核糖核酸序列。该12例患者的蛔虫粪检和血清学检查均呈阳性。结论部分原发性胆管结石内有蛔虫脱氧核糖核酸的存在,蛔虫感染可能对原发性胆管结石的形成有一定作用。

Objective To search for the molecular figngerprint of ascaris lumbricoides in intrahepatic stones. Methods In 56 cases deoxyribonucleic acid （DNA） was exstracted from intrahepatic stones and surgically obtained bile duct tissues, stool and sercum examinations were performed. Based on the sequence and designed primers of ITS-2 of ascaris lumbricoides, polymerase chain reaction （ PCR ） was performed. Results There were 12 positive results in 56 （21.4%） cases of intrahepatic stones. Positive reaction appeared on the examinations of the stool on ascaris lumbricoides and the blood serum among 12 cases. Conclusions Ascaris lumbricoides DNA was found in human of intrahepatic stones, indicating that ascaris lumbricoides infection may play a role in the formation of intrahepatic stones.