仿生型BG-COL-HYA-PS复合支架细胞相容性

The compatibility of Osteoblast on the biomimetic composite scaffold BG-COL-HYA-PS

目的:研究仿生型BG-COL-PS-HYA复合支架材料与成骨细胞的相容性。方法:将小鼠胚胎成骨细胞种植于BG-COL-HYA-PS、BG-COL复合材料、58SBG支架上,用MTT法、ALP活性测定等观察细胞在材料中的生长情况。通过体外实验方法,观察其生物相容性。结果:成骨细胞在BG-COL-HYA-PS材料上能良好粘附、增殖,而在58SBG材料上粘附差、细胞逐渐死亡。MTT法结果显示:联合培养后,BG-COL-HYA-PS组的OD值为0.314±0.004,5天时达到0.621±0.002,分别为58SBG组的1.49倍和1.44倍,P<0.05。结论:仿生型BG-COL-PS-HYA复合支架具有天然骨分级结构和有良好的生物相容性,在诱导成骨细胞增殖方面性能优越,可作为骨组织工程支架材料,临床应用前景广阔。

Objective： To explore the compatibility of osteoblast culturing on the biomimetic composite scaffold BG-COL-HYA-PS. Methods： Mouse embryo osteoblasts were seeded on the composite scaffold BG-COL-HYA-PS, BG-COL and bioactive glass 58SBG. respectively. MTT assay, ALP activity were performed to analyze the growth, function and compatibility of osteoblast. Results Osteoblasts adhered well on the BG-COL-HYA-PS, but poorly on 58SBG and even gradually died. MTT results showed that the OD value of the BG-COL-HYA-PS group was about 0.314±0.004 at the first day after seeding and reached 0.621±0.002 at the 5th day, which was higher about 1.49 and 1.44 time than those of the 58SBG group （P〈 0.05）. The structures of the biomimetic BG-COL-PS-HYA scaffold, possessing good biocompability, is resemble to that of natural bones. It is faverable for osteoinductivity and biological safety, and is suitable to be an ideal scaffold for tissue engineering.