摘要
AIM： To observe the dynamic changes of liver microcirculation in vivo after arterial embolization with degradable starch microspheres （DSM）. METHODS： DSM were injected into the proper hepatic artery through a silastic tube inserted retrogradely in gastroduodenal artery （GDA） of SD rats. Fluorescent microscopy was used to evaluate the dynamic changes of blood flow through the terminal portal venules （TPVs）, sinusoids and terminal hepatic venules （THVs）. The movements of DSM debris were also recorded. Six hours after injection of DSM, percentages of THVs with completely stagnant blood flow were recorded. RESULTS： Two phases of blood flow change were recorded. In phase one： after intra-arterial injection of DSM, slow or stagnant blood flow was immediately recorded in TPVs, sinusoids and THVs. This change was reversible, and blood flow resumed completely. In phase two： after phase one, blood flow in TPVs changed again and three patterns of blood flow were recorded. Six hours after DSM injection, 36.9% ± 9.2% of THVs were found with completely stagnant blood flow. CONCLUSION： DSM can stop the microcirculatory blood flow in some areas of liver parenchyma. Liver parenchyma supplied by arteries with larger A-P shunt is considered at a higher risk of total microcirculatory blood stagnation after injection of DSM through hepatic artery.
瞄准：观察肝的动态变化在有可能减解的淀粉的动脉的 embolization 以后的微发行量在活体内微范围(DSM ) 。方法：DSM 通过在 SD 老鼠的胃与十二指肠的动脉(GDA ) retrogradely 插入的一个 silastic 试管被注入合适的肝的动脉。荧光灯的显微镜学被用来通过终端门小静脉(TPV ) 评估血流的动态变化，窦状隙和终端肝的小静脉(THV ) 。DSM 碎片的运动也被记录。在 DSM 的注射以后的六个小时，有完全停滞的血流的 THV 的百分比被记录。结果：血流变化的二个阶段被记录。在阶段一：在 DSM 的 intra 动脉的注射以后，慢或停滞的血流立即在 TPV，窦状隙和 THV 被记录。这个变化是可逆的，并且血流完全恢复了。在阶段二：在阶段一以后，在 TPV 的血流再变化了，血流的三个模式被记录。在 DSM 注射以后的六个小时， 9.2% THV 与完全停滞的血流被发现的 36.9%+/- 。结论：DSM 能在肝实质的一些区域停止微循环血流。动脉与更大的 A-P 分流供应的肝实质通过肝的动脉在 DSM 的注射以后在全部的微循环的血停滞的更高的风险被考虑。
