Disordered beta-catenin expression and E-cadherin/CDH1 promoter methyiation in gastric carcinoma

瞄准：在胃的癌的原子核或膜 / 细胞质调查 beta-catenin 的分发房间，在 E-cadherin 基因甲基化之间的关系和它的表示，和象在预言肿瘤渗入的潜在的分子的标记的 beta-catenin 和 E-cadherin 的角色。方法：胃的癌的 29 个案例，分类同样弥漫、肠的变体，为学习被选择。原子、细胞质的蛋白质被净化， beta-catenin 内容被 ELISA 检测。E-cadherin/CDH1 基因倡导者的 DNA 甲基化被 methylation 特定的 PCR 和 compaired 学习， E-cadherin 表示由免疫组织化学检测了。结果：在胃的癌的 27 种情况中，在原子核和膜 / 细胞质之间的 beta-catenin 内容的比率与 T 分类被相关(r = 0.392， P = 0.043 ) 。意义在 T2 和 T3 组之间是在场的。没有关联以他们的 beta-catenin 分发在弥漫、肠的变体之间被检测。在胃的癌的弥漫的变体的 21 种情况中，在在 CDH1 基因甲醇化物组和甲醇化物得非的组之间的 E-cadherin 表示有差别(29 % 对 71 % ， P = 0.027 ) 。在 CDH1 基因甲基化和 T 分类之间的关联都没被发现，也不是在 E-cadherin 表示之间的意义，肿瘤渗入分级。结论：比较分析原子并且 membrane/cytoplasmic beta-catenin 能预言本地肿瘤渗入。E-cadherin/CDH1 基因甲基化是为它在弥漫的变体的基因沉默的一个重要原因胃的癌。当 E-cadherin 不在时的 CDH1 基因的甲基化是在胃的致癌作用的一个早事件。

AIM： TO investigate the distribution of beta-catenin in nuclei or membrane/cytoplasm of gastric carcinoma cells, the relationship between E-cadherin gene methylation and its expression, and the role of beta-catenin and E-cadherin as potential molecular markers in predicting tumor infiltration. METHODS： Twenty-nine cases of gastric carcinoma, classified as diffuse and intestinal variants, were selected for study. Nuclear and cytoplasmic proteins were purified and beta-catenin content was detected by ELISA. DNA methylation of E-cadherin/CDH1 gene promoter was studied by methylation-specific PCR and compaired with E-cadherin expression detected by immunohistochemistry. RESULTS： In 27 cases of gastric carcinoma, the ratio of beta-catenin content between nuclei and membrane/ cytoplasm was correlated with the T-classification （r = 0.392, P = 0.043）. The significance was present between T2 and T3 groups. No correlation was detected between diffuse and intestinal variants in terms of their betacatenin distribution. In 21 cases of diffuse variants of gastric carcinoma, there was a difference in E-cadherin expression between CDH1 gene-methylated group and non-methylated group （29 % vs 71%, P = 0.027）. No correlation between CDH1 gene methylation and T-classification was found, neither was the significance between E-cadherin expression and tumor infiltration grade. CONCLUSION： Comparative analysis of nuclear and membrane/cytoplasmic beta-catenin can predict local tumor infiltration. E-cadherin/CDH1 gene methylation is an important cause for its gene silence in diffuse variant gastric carcinoma. Methylation of CDH1 gene in the absence of E-cadherin is an early event in gastric carcinogenesis.