Genetic instability of BRCA1 gene at locus D17S855 is related to clinicopathological behaviors of gastric cancer from Chinese population

瞄准：在中国人口在地点 D17S855，和他们与胃的癌症的 clinicopathological 特征的关系调查基因 BRCA1 的基因不稳定性。方法：Microsatellite 不稳定性(MSI ) 和在地点 D17S855 的基因 BRCA1 的杂合现象(LOH ) 的损失在胃的癌症和相应非癌的胃的织物的 37 件样品之间被比较。结果：在地点 D17S855 的 MSI 在胃的癌症(18.95%) 的 37 件样品中的 7 个是积极的。MSI 与淋巴节点转移，组织学的类型或肿瘤区别与 TNM 阶段而是没有关系有一种靠近的关系。在 TNM 的 MSI 积极频率我 + II (31.58% ， 6/19 ) 在 TNM III + IV 比那高得多(5.56% ， 1/18 ) ，(P 【 0.05 ) 。LOH 积极的率是 18.92%(7/37 ) 。LOH 没在 TNM III + IV 有关系到组织学的类型，肿瘤区别或淋巴节点转移，而是 LOH 积极的率是 33.33%(6/18 ) ，在 TNM 的比那高的大部分我 + II (5.26% ， 1/19 ) ，(P 【 0.05 ) 。BRCA1 蛋白质在胃的癌症的 37 件样品中的 14 个被表示。在 TNM 的 BRCA1 蛋白质的积极的率我分别地， + II 和 TNM III + IV 是 57.89% 和 16.67%(P 【 0.05 ) 。BRCA1 蛋白质的积极的率在高区别样品是 77.78% ， 30.77% 在中间的区别并且 12.50% 在更低的区别样品，(P 【 0.05 ) 。结论：BRCA1 基因的 MSI 能在中国人口在分散的胃的癌症的早阶段被用作一个分子的标记。因此， LOH 发生在胃的癌症的以后的时期它能被用作预示因素。

AIM： To investigate genetic instability of gene BRCA1 at locus D17S855, and their relationship with clinicopathological characteristics of gastric cancer in Chinese population. METHODS： Microsatellite instability （MSI） and loss of heterozygosity （LOH） of gene BRCA1 at locus D17S855 were compared between 37 samples of gastric cancer and corresponding non-cancerous gastric tissue. RESULTS： MSI at locus D17S855 was positive in 7 of 37 samples of gastric cancer （18.95%）. MSI had a close relationship with TNM staging but no relation with lymph node metastasis, histological type or tumor differentiation. MSI positive frequency in TNM Ⅰ ＋ Ⅱ （31.58%, 6/19） was much higher than that in TNM Ⅲ＋ Ⅳ （5.56%, 1/18）, （P 〈 0.05）. LOH positive rate was 18.92% （7/37）. LOH had no relationship to histological type, tumor differentiation or lymph node metastasis, but LOH positive rate in TNM Ⅲ＋ Ⅳ was 33.33% （6/18）, much higher than that in TNM Ⅰ ＋ Ⅱ （ 5.26%, 1/19）, （P 〈 0.05）. BRCA1 protein was expressed in 14 of 37 samples of gastric cancer. The positive rates of BRCA1 protein in TNM Ⅰ ＋ Ⅱ and TNM Ⅲ＋ Ⅳ were 57.89% and 16.67%, respectively, （P 〈 0.05）. The positive rate of BRCA1 protein was 77.78% in high differentiation samples, 30.77% in middle differentiation and 12.50% in lower differentiation samples, （P 〈 0.05）. CONCLUSION： MSI of BRCA1 gene could be used as a molecular marker in early phases of sporadic gastric cancer in Chinese population. LOH occurs at later period of gastric cancer, therefore, it could be used as prognostic factor.