病理性瘢痕中S100蛋白基因的差异表达

Differential expression of S100 protein gene in pathological scar

目的:探讨S100蛋白基因的表达情况与病理性瘢痕形成的相关性。方法:实验于2003-05/2005-01在南方医科大学分子生物学研究所完成。病理性瘢痕标本来源于南方医院整形外科手术患者,正常皮肤组织来源于无瘢痕体质趋势及无免疫性疾病的包皮手术标本,患者均知情同意。增生性瘢痕组3例,瘢痕疙瘩组3例,正常皮肤组10例。抽提增生性瘢痕和瘢痕疙瘩与正常皮肤组织的总RNA,反转录并双色荧光标记,与含11个S100蛋白基因的人类基因表达谱芯片杂交,经荧光扫描和数据处理,进一步进行统计学分析,检测S100蛋白基因在3组组织中的表达变化。结果:与正常皮肤组织相比,11个S100蛋白基因在增生性瘢痕组织中差异表达具有显著性意义的基因有3个,S100A7,S100A8和S100A9均为上调表达;而瘢痕疙瘩组织中仅有S100A2基因的下调表达具有显著性意义。结论:①S100蛋白基因的差异表达与病理性瘢痕的形成密切相关。②S100蛋白基因成员在增生性瘢痕和瘢痕疙瘩中的差异表达有助于两者的鉴别诊断。③S100A2在瘢痕疙瘩中低表达而在增生性瘢痕中正常表达,可能与瘢痕疙瘩的恶性度高于增生性瘢痕,且能向周边正常组织浸润有关。

AIM： To investigate the correlation between expression of S100 protein gene and formation of pathological scar. METHODS： The experiment was conducted in the Institute of Molecular Biology, Southern Medical University from May 2003 to January 2005, Specimens were obtained from patients who received plastic surgery in Nanfang Hospital and normal skin tissues were obtained from specimens in prepuce operation, which had no keloid tendency or immune diseases, All patients knew and agreed with the items. Hyperplastic scar group （n=3）, keloid group （n=3）, normal skin group （n=10）, Total DNA of hyperplastic scar, keloid and normal skin were extracted, done with inverse transcript and labeled with two color fluorescence, and then crossed with human gene expression chips including 11 S100 protein genes, Fluorescent scanning and data processing were conducted for further statistical analysis, Changes of expression of S100 protein gene in three groups were detected. RESULTS： Compared with normal skin tissues, differential expressions of 3 genes in 11 S100 protein genes of hyperplastic scar tissues were significant. S100A7, S100A8 and S100A9 were in up-regulation, Only expression of S100A2 gene in the keloid group was in remarkable down-regulation, CONCLUSION： ①Differential expression of S100 protein gene is closely related to the formation of pathological scar,②S100 protein genes differentially express in hyperplastic scar and keloid, which facilitate the differential diagnosis between the two, ③Expression of S100A2 gene is lower in keloid and normal in hyperplastic scar, which may be relate to that malignancy of keloid is severer than that in hyperplastic scar and can infiltrate towards peripheral normal tissues.