TRAIL联合化疗或热疗抑制人肝癌细胞SMMC-7721体外增殖作用的研究

Research on combination of TRAIL with chemotherapy or hyperthermia against hepatocellular carcinoma cell line SMMC-7721 in vitro

目的:在国内外现有的研究基础上探讨数种化疗药物:顺铂、羟基喜树碱、丝裂霉素、健择或热疗联合肿瘤坏死因子相关凋亡诱导配体(tumornecrosisfac-tor-relatedapoptosis-inducingligand,TRAIL)体外抑制肝癌细胞增殖的效果,以确定联合方案是否具有协同效应。方法:使用MTT法测定单独或联合应用TRAIL及化疗药物羟基喜树碱、顺铂、丝裂霉素、健择或热疗对人肝癌SMMC-7721细胞的体外增殖的抑制作用,使用流式细胞仪检测不同方案对SMMC-7721细胞凋亡率的影响。结果:TRAIL联合应用4种化疗药物均可以显著提高化疗药物引起的细胞增殖抑制及细胞凋亡。以亚毒性剂量浓度的顺铂为例,对照组、化疗组、TRAIL组、TRAIL联合化疗组的细胞增殖抑制率分别为0、14·91%、17·92%和62·45%;细胞凋亡率分别为1·59%、3·38%、8·78%和27·58%。但TRAIL联合热疗并不表现出明显的协同作用。结论:联合应用TRAIL与化疗药物对SMMC-7721细胞体外增殖的抑制及诱导细胞凋亡具有显著的协同作用,联合应用TRAIL与热疗未表现出明显的协同作用。

OBJECTIVE： To evaluate whether concurrent exposure of human hepatocellular carcinoma cells SMMC-7721 to TRAIL with chemotherapeutic agents or hyperthermia will increase anticancer effects. METHODS： Four chemotherapeutic agents such as cisplatin, hydroxycamptothecin, mitomycin C and gemcitabine were applied. MTT assay was used for quantitative evaluation of proliferation of SMMC-7721 and cytotoxicity of anticancer drug. Flow cytometric analyses were used for the assessment of apoptosis rates after the treatment. RESULTS： The combination of the two treatments induced significantly higher apoptosis and cytotoxicity than exposure to TRAIL or chemotherapeutic agents alone, but the combination of TRAIL and hyperthermia did not show synergistic effect. CONCLUSIONS： While both TRAIL and chemotherapeutic agents can individually induce apoptosis and anti-proliferation effect, they may offer synergistic benefits when used concurrently.