姜黄素对代谢综合征大鼠血浆TNF-α和血清sICAM-1水平的影响

Effects of curcumin on TNF-α level in plasm and sICAM-1 level in serum of metabolic syndrome rats

目的探讨姜黄素对代谢综合征大鼠血浆TNF-α和血清sICAM-1水平的影响。方法雄性SD大鼠20只施行两肾一夹术后普通饲料喂养4周,诱发肾性高血压,继以高果糖饲料喂养4周,诱导建立代谢综合征模型。将造模成功的17只SD大鼠随机分为模型组(n=8),姜黄素组(200mg/kg/d)(n=9),另设假手术对照组(n=8)。姜黄素组直接灌胃给药,模型组和假手术组灌等量的生理盐水。用药6周后,检测血清学指标,以及用ELISA法测定血浆TNF-!和血清sICAM-1的蛋白水平,以胰岛素敏感指数判断机体胰岛素抵抗状态。结果模型组大鼠血浆TNF-α和血清sICAM-1水平明显高于正常对照组大鼠(P<0.05);姜黄素组大鼠血浆TNF-α和血清sICAM-1水平明显低于模型组大鼠(P<0.05)。结论姜黄素能明显降低代谢综合征大鼠血浆TNF-α和血清sICAM-1水平,改善胰岛素抵抗,阻止或延缓减慢动脉粥样硬化的发生。

Objective：To investigate the effects of curcumin on the levels of TNF-α in plasm and sICAM-1 in serum of metabolic syndrome rats. Method：Twenty-two adult male SD rats were operated on two kidneys,one-chipped to induce renal hypertension. After operation,the rats were fed with a common diet for 4 weeks,and subsequently a high fructose diet for 4 weeks to induce metabolic syndrome. The 18 surviving insulin resist syndrome rats were grouped randomly as MS control,cureumin（200mg/kg/d）.sham-operated group（n=8） were taken randomly as non-infraction control. Four weeks after treatment with the drugs by gastric garage. The levels of TNF-α in plasm and sICAM-1 in serum were measured by ELISA .Insulin resist of animal and human was assessed by ISI. Result： In comparing with that of the control group,the levels of TNF-α in plasm and sICAM-1 in serum were significantly increased in MS rats （P〈0.05）. The levels of TNF-α in plasm and sICAM-1 in serum of curcumin group were significantly decreased in contrast to that of the MS rats （P〈0.05）.Conclusion：Curcumin can reduce the levels of TNF-α in plasm and sICAM-1 in serum,also can improve the symptom of insulin resistance and stop or make the happening of atherosclerosis slower.