不同性别之间先天性核性白内障蛋白质2-D电泳的差异研究

Detection of the differences of lens proteins in the different gender of congenital nuclear cataract by 2-DE

目的应用双向电泳（two-dimensional electrophoresis，2-DE）技术，对比年龄匹配的不同性别核型先天性白内障蛋白质2．DE的表达，寻找差异的蛋白质。方法收集年龄匹配的核型先天性白内障患儿的混浊晶状体组织，采用固相pH梯度（IPG）等点聚焦2-DE技术进行蛋白质的分离，使用Image Master图像分析软件分析电泳图像，对比蛋白质表达的差异。结果大部分晶状体蛋白质分布在Mr14400～66000、等电点（PI）5～9的范围内，呈高丰度表达的蛋白质斑点分布在Mr20100～45000、等电点（PI）6～8的区域内。经软件分析，女性核性先天性白内障患儿识别出（452±23）个蛋白斑点，男性患儿组识别出（421±19）个蛋白斑点，将不同性别患儿的2．D结果进行匹配比较。发现有119个斑点的蛋白质表达量有变化：其中14个点蛋白质表达量减少200％以上，编码19号的蛋白质表达量减少了500％以上；其中7个蛋白斑点表达量增加了200％以上，编码384号的蛋白斑点增加350％以上。结论性别不同的核性先天性白内障患儿的蛋白质表达存在差异。

Objective To investigate the different expressed proteins in the different gender of congenital cataract with the matched years old by twodimensional electrophoresis（2-DE）. Methods The congenital nuclear cataract lens with matched years old were collected. The separation of lens proteins was made by immobilized pH gradients 2-DE. The different expressed proteins were analyzed by 2-DE imaging analyzer. Results The maps of 2-DE showed that major lens proteins were in the section of PI 5-9 with relative molecular weight of 14400 - 66000 , while more abundant lens proteins were in the section of PI 6- 8 with relative molecular weight of 20100- 45000. About （452 ± 23） prtein spots were detected by image masteranalysis software in female congenital nuclear cataract group and （421 ± 19） protein spots in male group. There were 119 protein spots different expression between two group. The expression of male lens protein compared with female decreased to more than 200% in 14 spots, in which spot No. 19 decreased to 500%. The expression of lens protein increased to more than 200% in 7 spots, in which spot No. 384 increased to 350%. Conclusion The expressed proteins in the congenital nuclear cataract lens are different between male and female with the roached old.