MMP-3和TIMP-1在白内障晶状体上皮细胞中的表达及意义

Expressions of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase-1 in lens epithelial cells of patients with cataract and their significance

目的探讨基质金属蛋白酶-3(matrixmetalloproteinase-3,MMP-3)和金属蛋白酶组织抑制因子-1(tissueinhibitorofmetalloproteinase-1,TIMP-1)与白内障发病机制的关系。方法采用免疫组织化学方法测定MMP-3和TIMP-1在前囊下性白内障31眼、核性白内障28眼和正常晶状体12眼上皮细胞(lensepithelialcell,LEC)中的表达。结果MMP-3在前囊下性白内障的表达分别与核性白内障、正常晶状体比较,差异有显著意义(χ2=31.490,34.479;P=0.000),而核性白内障与正常晶状体相比较,差异无显著意义(χ2=2.449,P=0.118);TIMP-1表达在3组LEC中差异均无显著意义(P>0.05);MMP-3与TIMP-1在前囊下性白内障LEC中的表达没有相关性(r=0.121,P=0.516),而在核性白内障中有相关性(r=-0.513,P=0.005)。结论MMP-3表达增强及MMP-3/TIMP-1的表达失衡可能与前囊下性白内障的形成密切有关。

Objective To investigate the expressions of matrix metalloproteinase-3（MMP-3） and tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） and the correlation between the both and the pathogenesis of cataract. Methods MMP-3 and TIMP-1 expressions in lens epithelial cells（LECs） of anterior subcapsular cataract （31 cases）, nuclear cataract（28 cases） and normal lens（12 cases） were detected by immunohistochemistry respctively. Results There was significant difference of expression of MMP-3 between anterior subcapsular cataract and nuclear cataract, normal lens （x^2 = 31. 490,34. 479; P = 0. 000） ; but there was no statistical significance between nuclear cataract and normal lens （x^2= 2. 449, P = 0. 118）. There was no significant difference of expression of TIMP-1 among three groups （P 〉 0.05）. No significant correlation was found between MMP-3 and TIMP-1 in LECs of anterior subcapsular cataract （ r = 0. 121, P= 0. 516） and significant correlation was found in nuclear cataract （ r =- 0.513,P= 0. 005）. Conclusion Augmentation expression of MMP-3 and imbalance between the expression of MMP-3 and TIMP-1 could play a critical role in the pathogenesis of anterior subcapsular cataract.