循环应力对构建组织工程软骨的影响

The effects of cyclic pressure on constructing of tissue engineering cartilage

目的探讨循环应力对体外构建组织工程软骨的影响。方法分离兔关节软骨细胞,体外扩增培养后种植于PGA无纺网支架上。实验组在循环应力下培养(0～200kPa,0.1Hz),对照组静态培养。实验组和对照组均培养4周。4周后取出组织工程软骨行大体标本观察。组织化学和免疫组织化学染色观察组织工程软骨结构以及Ⅱ型胶原分泌情况。结果体外培养4周时,实验组的组织工程软骨形态和色泽更加接近正常透明软骨,厚度和组织弹性优于对照组,实验组的软骨细胞数量和细胞外基质合成明显增加,分泌Ⅱ型胶原能力更强。结论循环应力刺激软骨细胞合成分泌Ⅱ型胶原增加,有利于在体外构建组织工程软骨。

Objective To study the effect of the cyclic pressure on the constructing of tissue engineering cartilage in vitro. Methods Free chondrocytes isolated from rabbit articular cartilage were seeded into PGA scaffolds after expansion in vitro. Experimental group was subjected to the cyclic pressure while the control group was cultured under normal condition. Both groups were cultured in vitro. After 4 weeks,the tissue engineering cartilage was observed. The type Ⅱ collagen was assessed by histological staining of HE and immunohistochemistrical stain from the tissue engineering cartilage. Results The tissue engineered cartilage under cyclic pressure is morphologically thicker and shows a higher extracellular matrix secretion and type Ⅱ collagen synthesis. Conclusion The cyclic pressure promotes chondrocytes to secrete type Ⅱ collagen, which benefits the construction of tissue engineering cartilage.