人血液荧光的光谱特性及其物质来源

Fluorescence Spectral Characteristics of Human Blood and Its Endogenous Fluorophores

取健康人的静脉血,首次实验测量了人血液的三维荧光光谱,并通过分析荧光激发-发射矩阵研究了血液自体荧光的物质来源。结果表明:人血液的荧光激发-发射对主要有260-630,280-340,340-460,450-520 nm,它们所对应的内源性荧光物质分别来源于血液中的内源性卟啉,色氨酸,还原烟碱腺嘌呤二核苷酸(磷酸盐)和黄素腺嘌呤二核苷酸。这些物质的荧光激发效率随着激发光波长的变化而表现出显著差异。

The three-dimensional fluorescence spectra and excitation-emission matrix of human blood were measured, and an attempt was made to exploit the endogenous fluorophores of major peaks in the UV and visible The result indicates that the absorption peaks of human blood appear at 274, 345, 415, 541 and 576 nm. Based on the analysis of fluorescence excitation-emission matrix, the major emission peaks of human blood occur at excitation-emission wavelength pairs of 260-630, 280-340, 340-460 and 450-520 nm, which are attributed to endogenous porphyrins, tryptophan, reduced nicotinamide adenine dinucleotide （NADH） or reduced nicotinamide adenine dinucleotide phosphate （NADPH）, and flavin adenine dinucleotide （FAD）, respectively. These results can be used to analyze and explain the effect of blood on the distortion of fluorescence signal of human tissues for optical diagnosis.