摘要
目的 对刺梨多糖进行分离纯化及神经营养活性研究。方法 采用DEAE-纤维素柱色谱及Sepharose CL-6B凝胶柱色谱对刺梨粗多糖进行分离纯化，得到RRTP-1～RRTP-8共8个多糖组分。凝胶柱色谱和聚丙烯酰胺凝胶电泳鉴定纯度。采用PC12细胞对各组分的神经营养活性进行筛选，并对纯化的单一多糖进行活性定量测定。结果 RRTP-1和RRTP-3为相对分子质量分布均一多糖。PC12细胞实验结果表明，多个多糖组分能刺激PC12细胞产生神经纤维样突起。结论 多数刺梨多糖组分具有神经营养活性。
OBJECTIVE To study the isolation and neurotrophic activity of polysaccharides from Rosa roxburghii Tratt.METHODS RRTP-1 - RRTP-8 were isolated by DEAF-cellulose and Sepharose CL-6B column chromatography from crude polysaccharide. Their homogeneity was determined by gel column chromatography and electrophoresis. Neurotrophic activity of RRTP-1 - RRTP-8 was screened by PC12 cell assay. Quantitative neurotrophie assays to homogeneous polysaeeharides were carried out.RESULTS RRTP-1 and RRTP-3 were homogeneous polysaccharides.The screening results showed that most of polysaccharide fractions induced the differentiation of PC12 cell. CONCLUSION The most of polysaccharide fractions from Rosa roxburghii Tratt have obvious neurotrophic activity.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第13期980-982,共3页
Chinese Pharmaceutical Journal
基金
贵州省优秀科技教育人才省长专项资金
关键词
刺梨
多糖
分离纯化
神经营养
神经生长因子
Rosa roxburghii Tratt
polysaccharide
isolation
neurotrophic activity
nerve growth factor
