血浆置换联合血液滤过治疗对增殖细胞核抗原表达的影响

Effect of plasma exchange combined with hemofiltration therapy on the expression of proliferating cell nuclear antigen

目的观察血浆置换联合血液滤过治疗对猪急性肝衰竭肝组织中增殖细胞核抗原(prol-iferatingcellnuclearantigen,PCNA)表达的影响。方法将中国实验小型猪12头随机分为两组,即对照组(6头)和治疗组(6头),两组均给予1.2g/kg体重的D-氨基半乳糖诱导建立药物性急性肝功能衰竭模型。治疗组于给药后24h及48h应用血浆置换联合血液滤过治疗,对照组未给予任何治疗。分别留取给药前、给药后6h、24h、48h、72h动物的肝穿组织,用免疫组织化学技术检测肝组织中PCNA的表达,观察比较肝衰竭过程中不同时间、治疗前后PCNA表达的变化。结果对照组动物肝组织中PCNA表达在模型建立成功后第6h即开始升高,48小时明显升高,此后又急剧下降。治疗组在治疗后PCNA表达明显高于对照组(P<0.05),差异有显著性。结论急性肝衰竭时,残存肝细胞仍有再生能力,血浆置换联合血液滤过治疗可以提高PCNA的阳性表达。

Objective To observe the effect of plasma exchange （PE） combined with hemofiltration （HF） on the expression of proliferating cell nuclear antigen （PCNA） in the livers of pigs with acute hepatic failure. Methods Twelve minipigs were divided into two groups randomly： Control group （n=6） and treatment group （n=6）. Both groups of animals were induced acute hepatic failure with administration of D-galactosamine （dosage, 1.2g/kg）.The treatment group was treated with PE combined with HF 24 hours and 48 hours later. The expression of PCNA in the liver tissue was detected on the 6th、 24th、 48th、 72nd hour by immunohistochemical staining. Results For the control group, the expression of PCNA appeared 6 hours later. The maximum expression of PCNA appeared on the 48th hour and then sharply declined. Compared with the control group, the expression of PCNA was markedly improved （P〈0. 05）, the difference was significant. Conclusion The residual hepatocytes of the pigs with acute hepatic failure can regenerate. PE combined with HF therapy can raise the expression of PCNA.