大鼠外周炎症组织内β-内啡肽的表达

EXPRESSION OF β-ENDORPHINE AT PERIPHERAL INFLAMED TISSUE OF RATS

目的:研究大鼠外周炎症组织中的β-内啡肽在炎症过程中表达的变化,为进一步探讨内源性阿片肽在外周炎症性疼痛中所起的作用。方法:在大鼠后足注射1%角叉菜胶产生外周炎症。分别在正常和炎症后6、12、24、72h取大鼠后足皮下组织,然后:①用HE染色后,观察炎症组织内的炎性细胞变化;②用免疫组织化学的方法检测大鼠后足皮下组织的β-内啡肽,并用图像分析系统进行半定量分析。结果:①在炎症过程,皮下组织中可见大量的白细胞浸润。②正常皮下组织无β-内啡肽表达。炎症6h大鼠足底皮下组织可见β-内啡肽表达,表达量在炎症24h到达最高峰,72h下降至6h水平。β-内啡肽免疫阳性的白细胞数在6、12、24h没有明显差异(P>0·05),72h后降低。结论:大鼠外周炎症组织中的β-内啡肽来源于浸润到炎症组织的白细胞,β-内啡肽表达量及含β-内啡肽免疫阳性的白细胞数随炎症过程变化而变化。

Objective：To observes source and changes of β-endorphine at peripheral inflamed tissue of rats, which is foundation for study analgesia of endorphine at peripheral inflamed tissue. Methods： Peripheral inflammation was induced by injected carrageenan to the hindpaws of the rat. Tissue samples were harvested 6, 12, 24 h and 72 h after injection of carrageenan. Subcutaneous paws tissue was obtained from the plantar. ①the change of infiltrated inflammatory cells in the paw tissue with HE Stained was observe. ②the β- endorphine in the subcutaneous paw tissue was identified by immunohistochemistry and half quantified by image analysis system. Result： ①There was a lot of infiltrating immune cells in the subcutaneous paw tissue during inflammation.② The-endorphine was not detected in the normal subcutaneous paw tissue. There was expression of-endorphine in subcutaneous paw tissue at 6th hour after injection of carrageenan. The amount of-endorphine was the highest in subcutaneous paw tissue at 24th hour during inflammation. There was not significantly difference at 6 h and 72 h. The number of β-endorphine-producing cells was not significantly different at 6,12,24 h during inflammation（ P 〈0.05）. But it decreased at 72 h. Conclusion：The β- endorphine in the inflammatory tissue comes from infiltrating leukocytes. The amount of β-endorphine and β-endorphine-producing cells change with inflammation.