人脑外伤后皮层基质金属蛋白酶9的表达:定量、定位和时相

Cortical expression of matrix metalproteinases-9 after human traumatic brain injury:quantity,Location and timing

目的探讨基质金属蛋白酶9（matrix metalloproteinases-9，MMP-9）在人创伤性脑损伤（traumatic brain injury，TBI）后挫伤皮层中的表达情况，包括表达位置、表达强度和表达时相。方法挫伤区皮层的标本来自于24位TBI患者，取样时间从伤后5h到5d，利用免疫组化技术测定MMP-9的表达强度。结果在人TBI后的挫伤区皮层中，MMP-9表达明显上调，表达高峰为伤后24-48h，MMP-9主要在神经元细胞中表达，神经胶质细胞中也有少量表达。结论MMP-9在人TBI后的挫伤区皮层中表达上调，提示其可能在TBI后的病理生理过程中起着重要作用。

Objective The aim of current study was to analyze the expression of matrix metalproteinases-9（MMP-9） in contused human brain tissue, and to identify the expressive location, quantitation and time points. Methods Contrustal brain tissue biopsies were obtained from 24 patients with surgery for brain from 5 hours to 5 days after trauma. The expression of MMP- 9 was detected by immunohistochemistry. Results The result showed an immediate, strong and persistent up-regulation of MMP-9 in the area surrounding the injured brain as early as 5 hours postinjury and was keeping at high level up to 5 days. The maximal expression of MMP-9 was detected at 24 to 48 hours post injury. Expression of MMP-9 was mainly located at neurons and slight expression in a few neuroglial cells. Conclusion The results suggest that MMP- 9 is up- regulate obviously in the early phase of human brain contusion. MMP-9 might play important roles in the pathophysiological course after TBI.