荧光分光光度法测定维生素B_2片的含量

Determination of Riboflavin Content in Vitamin B_2 Tablets by Fluorophotometer

目的：建立荧光分光光度法测定维生素B2（VB2）片剂的含量。方法：激发波长为464nm，发射波长为520nm。结果：VB2片剂在0．2—1．0mg／L浓度范围内，荧光强度与浓度呈良好线性关系，回收率为98．8％（n=5），RSD=2．32％。结论：该方法简便、准确，专属性强，试样量少，可用于对VB：片剂的质量控制。

Objective：To establish a method for the determination of riboflavin in Vitamin B2 tablets with fluorophotometer spectrometer . Method：The excitation and emission wavelengths are λEx = 464 nm and λEM = 520 nm respectively. Result： From 0.2 to 1.0 mg/L of vitamin B2 tablets, the intension of fluorescence is satisafactory. Conlusion ： The method was simple, accurate, it can be used for quality control of vitamin B2 tablets.