氯酚类化合物对厌氧产甲烷毒性的试验研究

Toxicity of Chlorophenols to Anaerobic Methane Formation

在（35±1）℃厌氧条件下，以葡萄糖为其基质，采用向歇试验法，通过测定甲完累计产量，研究了2，4-二氯酚（2，4-DCP）、2、4、6-三氯酚（2、4、6-TeCP）及五氯酚（PCP）的产甲烷毒性，利用相对话性值判断氯酚类化合物对产甲烷菌的抑制程度，试验结果表明；2、4、6-DCP浓度小于20mg/L时没有对产甲烷菌活性产生抑制作用，当浓度在40-60mg/L时为轻度抑制；2、4、6-DCP浓度为5mg/L时为轻度抑制，浓度在10-60mg/L时为中度抑制；2、4、6-DCP浓度在50-10mg/L时为轻度抑制剂浓度在20-60mg/L时为中度抑制；PCP浓度在5-40mg/L时为中度抑制，浓度为60mg/L时为重度抑制；四种氯本分对产甲烷菌活性的抑制程度大小顺序为：PCP〉2,4,6-TCP〉2,3,4,6-TeCP〉2,4-DCP.

A batch anaerobe test was conducted to examine toxicity of 2,4-dichlorophenol（2,4-DCP）,2、4、6-Trichlorophenol（2,4,6-TCP）,2,3,4,6-Tetrachlorophenol（2,3,4,6-TeCP）and pentachlorophenol（PCP）to the methanc formation throngh measuring accumulative methane production.The relative activity values were used to judge the inhibition level of chlorophenols on methanogenic bacteria.The test conditions was as follows：glucose was used as co-substrate and the temperature was（35±1）℃.It didn＇t eause inloibition when concentration of 2,4-DCPwas below 20mg/L ,Slight inhibition was caused when concentration of 2,4-DCP,2,4,6-TCP and 2,3,4,6-TeCP were 40-60mg/L,5mg/L and 5-10mg/L respectively.When concentration of 2,3,4,6-TCP,2,3,4,6-TeCP and PCP were 10-60mg/L,5mg/L and 5-10mg/L respectively.When concentration of 2,3,4,6-TCP,2,3,4,6-TeCP and PCPwere 10-60mg/L,20-60mg/L and 5-40mg/L respectively,middle inbibition was observed.The activity was strongly inhibited when PCP concentration increased to 60mg/L.the seqnence of inhibition activitied was PCP〉2,4,6-TCP〉2,3,4,6-TeCP〉2,4,-DCP.