增强型电荷耦合器件用于毛细管电泳激光诱导荧光的检测

Laser-Induced Fluorcence-Intensified Charge Coupled-Device(LIF-ICCD) Used for Detection of Capillary Electrophoresis

增强型电荷耦合器件用于毛细管电泳激光诱导荧光的检测马明生，吴晓军，刘国诠（中国科学院化学研究所，北京，１０００８０）关键词增强型电荷耦合器件，激光诱导荧光，毛细管电泳，异硫氰酸荧光黄近年来，毛细管电泳技术在生化分离、分析中得到广泛的应用。目前，毛细管...

Laser-induced fluorescence-intensified charge coupled-device(LIF-ICCD)was applied as detection of capillary electrophoresis.Various effects were studied both for the data collection of frequency and sensitivity of fluorescence detection in order to arrive at the optimun conditions.A capillary electrophoregram of 4.5 ×10-12 mo1/L of fluorescein isothiocyanate(FITC)was obtained with the LIF-ICCD detector under the chosen conditions.