摘要
利用荧光测活法比较了HRP在有机相与水相胶束体系中催化不同芳香胺类的动力学常数,发现在水相胶束体系中,HRP是在一个较严格的亲、疏水界面进行催化反应。同时对界面酶学性质进行了初步研究,讨论了在胶束中不同增溶位置对反应动力学常数的影响。
The kinetic constants of different aromatic amines substrates catalyzed by HRP in the organic solvent and aqueous micelle system were compared by the method of fluorescence,It was found that the catalytic reactions by HRP in the aqueous micelle system took place on a strict hydrophilic/hydrophobic interface;Meanwhile,the enzymatic properties on the interface were preliminarily studied and the effect of the substrates with different extending solvent sites in the micelle on the reaction kinetic constants was discussed.
出处
《高等学校化学学报》
CSCD
北大核心
1996年第12期1927-1929,共3页
Chemical Journal of Chinese Universities
基金
吉林大学酶工程开放实验室基金
关键词
辣根过氧化物酶
水相胶束
反应微环境
酶
Horseradish perosidase
Aqueous micelle
Reaction microenvironment