现场光化学荧光探针法测定DNA──以9，10－蒽醌－2－磺酸钠为光化学荧光探针

Determination of DNA by Use of in situ Photochemical Fluorescence Probe──Sodium 9,10-Anthraquinone-2-sulfonate as a Photochemical Fluorescence Probe

现场光化学荧光探针法测定ＤＮＡ──以９，１０－蒽醌－２－磺酸钠为光化学荧光探针李文友，许金钩，郭祥群，朱庆枝，赵一兵（材料和生命过程分析科学开放研究实验室，厦门大学化学系，厦门，３６１００５）关键词ＤＮＡ，光化学荧光探针，９，１０－蒽醌－２－磺酸钠光...

An in situ photochemical fluorescence probe method for the determination of DNA with sodium 9,10-anthraquinone-2-sulfonate(AQS)as a photochemical fluorescence probe was developed for the first time.It was based on the conversion of AQS into an intensively fluorescent product on irradiating with UV radiation.The photochemical reaction is decelerated by DNA.The determination can be carried out by measuring the fluorescence intensity at a fixed time.The calibration graph was linear in the range 0～80 ng/mL CT DNA(r=0.9991), the limit of detection was 3.2 ng/mL CT DNA(n=9).The kinetic behaviour of the photochemical reaction and the effects of some experiment conditions were investigated and discussed in detail.The results of absorption spectra experiments and competitive binding experiments suggest that the interaction between AQS and DNA may be intercalative.