太白楤木对成纤维细胞增殖及功能的影响

Effects of Aralia chinesis L on Proliferation and Function of Fibroblasts

目的探讨太白楤木对成纤维细胞增殖、活力及对培养上清中透明质酸(HA)产生的影响,研究太白楤木抗肝纤维化机制。方法采用体外培养的NIH3T3(成纤维细胞)作为肝星状细胞(HSC)的替代模型,常规培养,太白楤木中药血清作用于细胞,用MTT法检测太白楤木中药血清对NIH3T3细胞增殖的影响;用放射免疫法测定培养上清中HA的生成。结果太白楤木对细胞无明显毒性,5%浓度太白楤木中药血清可显著抑制细胞增殖(P<0.01),降低NIH3T3细胞培养上清中HA含量,与对照组比较差异显著(P<0.05)。结论太白楤木中药血清可显著抑制NIH3T3细胞增殖和HA的合成,在体外具有一定的抗肝纤维化作用。

Objective To study the effects of Aralia chinesis on the proliferation and viability of fibroblasts and on the production of the hyaluronic acid （HA） in the cultured supernatant, and to explore its anti- hepatofibrosis mechanism. Methods NIH3T3 fibroblasts, which were cultured in vitro by routine method and were used as the substitutive model of hepatic stellate ceils （HSC）, were cultured with the rats serum containing Aralia chinese. The effects of Aralia chinesis serum on the cell proliferation was measured by methabenzthiazuron （MTT） assay and the HA content in cultured supernatant was detected by radioimmunoassay. Results Aralia chinesis serum showed no significant toxicity on NIH3T3 fibroblasts. 5 % concentration serum of Aralia chinesis inhibited the cell proliferation and the synthesis of HA significantly （P 〈 0.01 ）, the content of HA in culture medium was decreased, which differed significantly from those in control group （ P 〈 0.05）. Conclusion The serum of Aralia chinesis can significantly inhibit NIH3T3 fibroblasts proliferation and HA synthesis, and play a certain role in anti - hepatofibrosis in vitro.