正交试验优选紫草提取工艺

Optimization of Extraction Process of Arnebia euchroma(Royle) Johnst. by Orthogonal Design

目的以紫草中总萘醌和左旋紫草素的含量为指标,研究超声萃取和索氏提取2种工艺方法在萃取紫草中有效成分的差异。方法通过正交试验安排实验条件,采用分光光度法测定紫草提取液中总萘醌含量,HPLC法测定提取液中左旋紫草素含量,比较超声萃取与索氏提取的提取效率,优选两种工艺方法的最佳工艺条件。结果2种工艺方法中,粒度对总萘醌的提取率均有非常显著影响,粒度较大的提取率高,但对紫草中左旋紫草素的提取率无显著影响;溶剂乙醇在2种工艺方法中表现出不同影响,超声萃取工艺乙醇的提取率较高,呈显著影响,而在索氏提取工艺中提取率较低,也呈显著影响。结论比较2种工艺,超声萃取具有高效、省时、节能的特点,优于索氏提取工艺。

Objective Using the contents of naphthaquinine and shikonin as the indices, the influences of ultrasonic extraction and soxhlet extraction on the active constituents in Arnebia euchroma（Royle） Johnst. were studied. Methods An orthogonal design was applied. Naphthaquinine content was determined by spectrophotometry and shikonin content by HPLC. The extraction rate of the two extracting methods was compared to optimize the process condition. Results By using the two extracting methods, particle size had an obvious effect on the extraction rate of naphthaquinine （the bigger particle, the higher extraction rate）, but had no effect on the extraction rate of shikonin; the solvent of ethanol showed different effects on the extraction rate of active constituents by using the two extraction methods, the extraction rate being higher by ultrasonic extraction while lower by soxhlet extraction. Conclusion Ultrasonic extraction is efficient, and with energy and time saving in extracting activeconstituentsofArnebiaeuchroma（Royle） Johnst. , which is superior to soxhlet extraction.