太原地区孕妇HBV感染者a决定簇的变异

Mutations of HBV a determinant cluster in pregnant women infected by HBV

目的 了解太原地区孕妇乙型肝炎病毒（HBV）感染者HBVS基因a决定簇变异情况。方法 2001年11月～2004年7月，采集太原市传染病医院妇产科住院的HBsAg阳性孕妇外周血，采用ELISA法检测血清HBsAg、HBeAg，巢式PCR法检测血清HBVDNA，对41例孕妇标本进行了PCR产物直接测序，与Genebank中HBV中国大陆流行株进行多重序列比对，分析HBVS基因a决定簇变异情况。结果 14．63％（6／41）孕妇携带HBVS基因a决定簇变异株。检出的突变位点中，aa126，aa127，aa131，aa143为较常见的氨基酸变异位点，aa125，aa135，aa137及aa140变异报道较少，大多数位点的变异导致了氨基酸替换。结论 太原地区孕妇HBV感染者携带a决定簇突变株。

Objective To explore mutation status in the Hepatitis B virus（HBV）S gene a determinant cluster of pregnant women infected by HBV in Taiyuan. Methods Sera were collected from HBsAg positive pregnant women in Taiyuan infectious hospital between Nov, 2001 and Jul, 2004. HBsAg and HBeAg in sera were tested by ELISA; HBV DNA in sera were tested by nested-PCR. Viral nucleotide sequences in 41 samples were determined by PCR-directed nucleotide sequencing. HBV S gene mutations were analyzed through multiple sequence alignment with sequences of HBV prevalence strains from China in GENBANK. Results 6（ 14.63 % ） out of 41 sera of pregnant women were testified carrying HBV S gene a determinant cluster variant strains. Amino acid mutant points-aa126, 127,131, and 143 were often reported. But the mutations in aa125, 135,137 and 140 were scarce. Amino acid substitutions took place in most positions. Conclusion Pregnant women infected by HBV in Taiyuan carried a determinant cluster variant strains.