苯致小鼠淋巴细胞DNA、RNA损伤作用

Damage effect of benzene on DNA and RNA of lymphocytes in mice in vivo

目的研究苯对小鼠淋巴细胞DNA、RNA损伤作用。方法采用单细胞凝胶电泳方法和3H-胸腺嘧啶1、4C-尿嘧啶掺入技术检测不同剂量苯[100,200,400 mg/(kg.bw)]经腹腔注射染毒对小鼠外周血、脾脏、胸腺淋巴细胞DNA、RNA损伤情况。结果除了苯染毒为100 mg/(kg.bw)剂量组外,小鼠外周血淋巴细胞DNA彗星细胞率、彗星尾长均高于阴性对照组(P<0.05),3H-胸腺嘧啶和14C-尿嘧啶掺入计数每分钟衰变数(dpm)值明显低于阴性对照组(P<0.01)。结论苯可致外周血淋巴细胞DNA损伤,明显影响小鼠脾脏、胸腺淋巴细胞DNA和RNA合成而产生遗传毒性作用。

Objective To explore the effect of exposure of benzene on DNA and RNA damage of lymphocytes in mice. Methods The single cell gel electrophoresis （SCGE） assay and the assay of radioactive compounds（3^H - thymidine and 14^C - Uridine） incorporation were used to detect the effect on DNA and RNA damage of peripheral blood, spleen and thymic lymphocytes in mice were intraperitoneally injected with benzene at various doses. Results Except for the 100 mg/kg dose, the rate of cells DNA with tail and the mean values of DNA tail length of peripheral blood lymphoeytes by exposure to benzene in mice were significantly higher than that of the control group, the 3^H - thymidine and 14^C - Uridin incorporation disintegrations per minute （dpm） of DNA and RNA by exposure to benzene in mice were significantly lower than that of the control group. Conclusion Benzene could cause DNA damage of peripheral blood lymphoeytes and significantly affect DNA and RNA synthesis of spleen and thymic lymphocytes in mice.